ArticleNature communications2022
Extracellular release of two peptidases dominates generation of the trypanosome quorum-sensing signal.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Glycerol metabolism triggers trypanosome differentiation into transmissible forms in mammalian tissue-like conditions.Nature communications · 2026Article
- Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia.Nature communications · 2025Article
- Membrane proteome analysis identifies key components of sensing in Phytophthora parasitica zoospores.Scientific reports · 2025Article
- Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.PLoS pathogens · 2025Article
- Mechanisms of life cycle simplification in African trypanosomes.Nature communications · 2024Article
- Depolymerization of SUMO chains induces slender to stumpy differentiation in T. brucei bloodstream parasites.PLoS pathogens · 2024Article
- Pathogenicity and virulence of African trypanosomes: From laboratory models to clinically relevant hosts.Virulence · 2023Review
- Cell-cell communication in African trypanosomes.Microbiology (Reading, England) · 2023Review
- Extracellular Vesicles Derived from Early and Late StageJournal of clinical medicine · 2022Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Trypanosomes causing African sleeping sickness use quorum-sensing (QS) to generate transmission-competent stumpy forms in mammalian hosts. This density-dependent process is signalled by oligopeptides that stimulate the signal transduction pathway leading to stumpy formation. Here, using mass spectrometry analysis, we identify peptidases released by trypanosomes and, for 12 peptidases, confirm their extracellular delivery. Thereafter, we determine the contribution of each peptidase to QS signal production using systematic inducible overexpression in vivo, and confirm this activity operates through the physiological QS signalling pathway. Gene knockout of the QS-active peptidases identifies two enzymes, oligopeptidase B and metallocarboxypeptidase 1, that significantly reduce QS when ablated individually. Further, combinatorial gene knockout of both peptidases confirms their dominance in the generation of the QS signal, with peptidase release of oligopeptidase B mediated via an unconventional protein secretion pathway. This work identifies how the QS signal driving trypanosome virulence and transmission is generated in mammalian hosts.
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