ArticleNature communications2022
Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Advances in engineering and applications of synthetic phase-separated membraneless organelles in biotechnology.Synthetic and systems biotechnology · 2026Review
- Review
- Light-Controlled Membrane Fusion in Synthetic Cells.Life (Basel, Switzerland) · 2026Review
- Engineered membraneless organelles inSynthetic and systems biotechnology · 2025Article
- Programming cellular condensates for living materials using an intrinsically disordered protein display platform.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Programmable solid-state condensates for spatiotemporal control of mammalian gene expression.Nature chemical biology · 2025Article
- Singlet Oxygen in Food: A Review on Its Formation, Oxidative Damages, Quenchers, and Applications in Preservation.Antioxidants (Basel, Switzerland) · 2025Review
- Article
- Cancer Photodynamic Therapy Enabled by Water-Soluble Chlorophyll Protein.ACS applied materials & interfaces · 2025Article
- Chlorophyll-Based Optogenetics to Control Membraneless Organelles.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Spatiotemporal Regulation of Cell Fate in Living Systems Using Photoactivatable Artificial DNA Membraneless Organelles.ACS central science · 2024Article
- Liquid-Liquid Phase Separation-Mediated Photocatalytic Subcellular Hybrid System for Highly Efficient Hydrogen Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A Minispidroin Guides the Molecular Design for Cellular Condensation Mechanisms inACS synthetic biology · 2023Article
- Rational design of phase separating peptides based on phase separating protein sequence of p53.Scientific reports · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Membraneless organelles (MLOs) formed via protein phase separation have great implications for both physiological and pathological processes. However, the inability to precisely control the bioactivities of MLOs has hindered our understanding of their roles in biology, not to mention their translational applications. Here, by combining intrinsically disordered domains such as RGG and mussel-foot proteins, we create an in cellulo protein phase separation system, of which various biological activities can be introduced via metal-mediated protein immobilization and further controlled by the water-soluble chlorophyll protein (WSCP)-a remarkably stable, red-light-responsive singlet oxygen generator. The WSCP-laden protein condensates undergo a liquid-to-solid phase transition on light exposure, due to oxidative crosslinking, providing a means to control catalysis within synthetic MLOs. Moreover, these photoresponsive condensates, which retain the light-induced phase-transition behavior in living cells, exhibit marked membrane localization, reminiscent of the semi-membrane-bound compartments like postsynaptic densities in nervous systems. Together, this engineered system provides an approach toward controllable synthetic MLOs and, alongside its light-induced phase transition, may well serve to emulate and explore the aging process at the subcellular or even molecular level.
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