Evidence map›Paper›PMID 35679863›Full record

ArticleDevelopmental cell2022

EED is required for mouse primordial germ cell differentiation in the embryonic gonad.

Matthew G Lowe, Ming-Ren Yen, Fei-Man Hsu, Linzi Hosohama, Zhongxun Hu, Tsotne Chitiashvili, Timothy J Hunt, Isaac Gorgy, Matthew Bernard, Sissy E Wamaitha and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Epigenetic priming in the male germline.Current opinion in genetics & development · 2024
    Review
  11. Article
  12. Article
  13. Epigenetic programming in the ovarian reserve.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023
    Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Matthew G LoweDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA.
Ming-Ren YenInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
Fei-Man HsuDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA.
Linzi HosohamaDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Zhongxun HuDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Tsotne ChitiashviliDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Department of Biological Chemistry, University of California, Los Angeles, CA 90095, USA.
Timothy J HuntDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Isaac GorgyDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Matthew BernardDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.
Sissy E WamaithaDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA.
Pao-Yang ChenInstitute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.
Amander T ClarkDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, CA 90095, USA; Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA 90095, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90095, USA. Electronic address: clarka@ucla.edu.
University of California, Los Angeles · USInstitute of Plant and Microbial Biology, Academia Sinica · TWBroad Center · US

Funding

UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Understanding Epigenetic Remodeling in Primordial Germ CellsR01HD058047 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CLARK, AMANDER · 2009 to 2023
$5.6M
NICHD NIH HHS R01 HD058047NIGMS NIH HHS T32 GM007185
6 · The paper itself

Abstract

Development of primordial germ cells (PGCs) is required for reproduction. During PGC development in mammals, major epigenetic remodeling occurs, which is hypothesized to establish an epigenetic landscape for sex-specific germ cell differentiation and gametogenesis. In order to address the role of embryonic ectoderm development (EED) and histone 3 lysine 27 trimethylation (H3K27me3) in this process, we created an EED conditional knockout mouse and show that EED is essential for regulating the timing of sex-specific PGC differentiation in both ovaries and testes, as well as X chromosome dosage decompensation in testes. Integrating chromatin and whole genome bisulfite sequencing of epiblast and PGCs, we identified a poised repressive signature of H3K27me3/DNA methylation that we propose is established in the epiblast where EED and DNMT1 interact. Thus, EED joins DNMT1 in regulating the timing of sex-specific PGC differentiation during the critical window when the gonadal niche cells specialize into an ovary or testis.

Indexed as

Germ CellsHistonesPolycomb Repressive Complex 2AnimalsCell DifferentiationDNA MethylationEctodermFemaleGonadsMaleMiceEed protein, mouseHistonesPolycomb Repressive Complex 2DNMT1EEDembryoH3K27me3meiosisovary developmentPRC2primordial germ cellstestis development

Identifiers

PMID35679863
PMCPMC9278986
OpenAlexW4282942440

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.