Evidence map›Paper›PMID 35679308›Full record

ArticlePloS one2022

Quality assurance practices in tuberculosis diagnostic health facilities in Ethiopia.

Yeshiwork Abebaw, Abebaw Kebede, Kirubel Eshetu, Ephrem Tesfaye, Mengistu Tadesse, Waganeh Sinshaw, Misiker Amare, Dinka Fikadu Gamtesa, Betselot Zerihun, Melak Getu and 3 more

Abstract read
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In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yeshiwork AbebawEthiopian Public Health Institute, Addis Ababa, Ethiopia.ORCID 0000-0001-6039-3430
Abebaw KebedeEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Kirubel EshetuEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Ephrem TesfayeEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Mengistu TadesseEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Waganeh SinshawEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Misiker AmareEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Dinka Fikadu GamtesaEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Betselot ZerihunEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Melak GetuEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Getachew SeidEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Anteneh YalewEthiopian Public Health Institute, Addis Ababa, Ethiopia.
Getu DiribaEthiopian Public Health Institute, Addis Ababa, Ethiopia.ORCID 0000-0002-0166-682X

Funding

Ethiopia-Emory TB Research Training ProgramD43TW009127 · FIC · EMORY UNIVERSITY · PI HENRY M BLUMBERG, Kidist Bobosha · 2013 to 2026
$3.4M
FIC NIH HHS D43 TW009127
6 · The paper itself

Abstract

introductionThe quality of tuberculosis laboratory services in health facilities is a mandatory component of detecting active pulmonary TB cases and treatment follow-up. However, ensuring the quality of laboratory test results is a concern. This study aimed to assess the quality assurance practices in the tuberculosis diagnostic health facilities of Ethiopia. MATERIALS AND

methodsA cross-sectional study was conducted from October 2018 to March 2019 at nine governmental TB-culture laboratories and 34 randomly selected GeneXpert® MTB/RIF (Xpert® MTB/RIF) testing health facilities in Ethiopia. Participating health facilities were interviewed and laboratory documents and records present since 2017 were observed. Prior to the data collection, training was given to the data collectors. Descriptive statistics were used to produce results and were presented with tables and graphs.

resultsFrom a total of 34 Xpert® MTB/RIF testing laboratories, 50% run Internal Quality Control (IQC) for Acid-Fast Bacillus (AFB) Microscopy and 67.6% had lot-to-lot verification of staining reagents. For the Xpert® MTB/RIF assay, a lot-to-lot verification of cartridge and method validation was performed only in 8.8%and 20.6% of Xpert® MTB/RIF testing laboratories respectively. All TB-culture laboratories included in the study ran negative control (start and end IQC) during TB-culture sample processing and performed lot-to-lot verification for Mycobacteria Growth Indicator Tube (MGIT) in 88.9% of TB-culture laboratories. External Quality Assessment (EQA) Proficiency Testing (PT) for AFB microscopy is practiced in 79.4% Xpert® MTB/RIF testing laboratories and 100.0% for the Xpert® MTB/RIF assay. TB-Culture PT participation practice among TB-culture laboratories was 88.9%. A major challenge for health facilities during PT participation was the AFB PT-sample transportation delay (40.7%) and the Xpert® MTB/RIF assay EQA-PT feedback missing (38.2%).

conclusionThis assessment reveals that IQC for AFB microscopy, lot-to-lot verification, method validation, and equipment calibration were not well-practiced. The majority of TB diagnostic health facility laboratories had EQA-PT participation practice, but a significant gap in PT-sample transportation and missing feedback was identified.

Indexed as

Mycobacterium tuberculosisTuberculosisCross-Sectional StudiesEthiopiaHealth FacilitiesHumansSensitivity and SpecificitySputum

Identifiers

PMID35679308

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.