Evidence map›Paper›PMID 35679015›Full record

ArticleApplied biochemistry and biotechnology2022

Cloning and Expression of Heparinase Gene from a Novel Strain Raoultella NX-TZ-3-15.

Yinyin Li, Yue Lin, Yingzi Jiang, Hafiza Mahreen Mehwish, Muhammad Shahid Riaz Rajoka, Liqing Zhao

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Yinyin Li *College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, Guangdong, China.
Yue Lin *College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, Guangdong, China.
Yingzi JiangCollege of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, Guangdong, China.
Hafiza Mahreen MehwishHealth Science Center, Shenzhen University, Shenzhen, 518060, China.
Muhammad Shahid Riaz RajokaLaboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, 980-8572, Japan. shahidrajoka@yahoo.com.
Liqing ZhaoCollege of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen, 518060, Guangdong, China. lqzhao@szu.edu.cn.
Shenzhen University · CNTohoku University · JP

Funding

Special Foundation for the Development of Strategic Emerging Industries of Shenzhen JCYJ20190808145613154Special Foundation for the Development of Strategic Emerging Industries of Shenzhen KQJSCX20180328100801771
6 · The paper itself

Abstract

Heparin is a class of highly sulfated, acidic, linear, and complex polysaccharide that belongs to the heparin/heparan sulfate (HS) glycosaminoglycans family. Enzymatic depolymerization of heparin by heparinases is a promising strategy for the production of ultra-low molecular weight heparins (ULMWHs) as anticoagulants. In the present study, a novel heparinase-producing strain Raoultella NX-TZ-3-15 was isolated and identified from soil samples. Herein, the heparinase gene MBP-H1 was cloned to the pBENT vector to enable expression in Escherichia coli. The optimized conditions made the activity of recombinant heparinase reach the highest level (2140 U/L). The overexpressed MBP-H1 was purified by affinity chromatography and a purity of more than 90% was obtained. The condition for biocatalysis was also optimized and three metal ions Ca

Indexed as

Heparan SulfatePolysaccharide-LyasesAnticoagulantsCloning, MolecularEscherichia coliGlycosaminoglycansHeparinHeparin, Low-Molecular-WeightHeparin LyaseSoilAnticoagulantsGlycosaminoglycansHeparan SulfateHeparinHeparin, Low-Molecular-WeightHeparin LyasePolysaccharide-LyasesSoilCloning and expressionHeparinHeparinase geneRaoutella NX-TZ-3–15

Identifiers

PMID35679015
OpenAlexW4281811541

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.