ArticleThe plant pathology journal2022
Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set.
Article in The plant pathology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 14 citations in OpenAlex.
- Development of rapid and visual Erwinia amylovora detection technology using recombinase polymerase amplification (RPA) combined with lateral flow dipstick (LFD).Pest management science · 2026Article
- Systematic Characterization ofInsects · 2026Article
- Evaluation of pathogenicity variation between twoFrontiers in microbiology · 2025Article
- Comprehensive assessment ofFrontiers in plant science · 2025Article
- Friends and Foes: Bacteria of the Hydroponic Plant Microbiome.Plants (Basel, Switzerland) · 2024Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.
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Registered trials
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