ArticleSkin health and disease2022
Ex vivo confocal microscopy for surgical margin assessment: A histology-compared study on 109 specimens.
Article in Skin health and disease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
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Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 25 citations in OpenAlex.
- The Diagnostic Accuracy of Ex Vivo Confocal Laser Scanning Microscopy for Basal Cell Carcinoma: A Systematic Review and Meta-analysis.Acta dermato-venereologica · 2026Pooled it
- Current role of HistologPathologica · 2026Pooled it
- Intraoperative Ex Vivo Confocal Microscopy for Surgical Margin Assessment in Squamous Cell and Basal Cell Carcinoma (HISTOBLOC): Protocol for a Prospective Monocentric Pilot Study.JMIR research protocols · 2026Article
- Diagnostic accuracy of two-photon fluorescence microscopy in Mohs surgery of basal cell carcinomas.Journal of the American Academy of Dermatology · 2026Article
- Emerging In Vivo and Ex Vivo Optical Imaging Technologies in Dermatology and Dermatopathology.Dermatopathology (Basel, Switzerland) · 2026Review
- Article
- Feasibility and workflow analysis of confocal microscopy in robot-assisted prostatectomy.BJU international · 2026Article
- Article
- Precision Diagnosis in Cutaneous Head and Neck Squamous Cell Carcinoma.Biomedicines · 2026Review
- Laser Confocal Microscopy May Be a Useful Tool in Neuropathological Intraoperative Examination.Diagnostics (Basel, Switzerland) · 2025Article
- Diagnostic Accuracy of Ex Vivo Confocal Microscopy for Surgical Margin Assessment of High-Risk Nodular Basal Cell Carcinoma.Cancers · 2025Article
- Vacuum-based specimen holder for rapid surgical specimen imaging.Biomedical optics express · 2025Article
- Article
- Confocal Laser Microscopy for Intraoperative Margin Assessment in Breast-Conserving Surgery: A New Procedure in the Pathology Laboratory Workflow.The American journal of surgical pathology · 2025Article
- Rapid On-Site Digital Histopatholocal Evaluation (RODE) on Endobronchial Ultrasound (EBUS) Mediastinal Lymph Node Cryobiopsy Sampling Using Confocal Laser Microscopy-A Proof-Of-Concept Study.Respirology case reports · 2025Article
- Breast tissue imaging atlas using ultra-fast confocal microscopy to identify cancer lesions.Virchows Archiv : an international journal of pathology · 2025Article
- Perspective on the use of fluorescence molecular imaging for peripheral and deepJournal of biomedical optics · 2025Review
- Review
- Article
- SENOSI Confocal Microscopy: A New and Innovating Way to Detect Positive Margins in Non-Palpable Breast Cancer?Life (Basel, Switzerland) · 2024Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: The assessment of surgical margins is mandatory to prevent local recurrence or distant dissemination of skin cancers. Histological examination of haematoxylin and eosin (H&E)-stained slides from paraffin-embedded or frozen samples is the gold standard for margin assessment, but is a time-consuming procedure. Ex vivo confocal laser scanning microscopy (CLSM) is an upcoming technique that scans unfixed fresh tissue rapidly, allowing fast per-operative margin assessment. Objective: Here, we propose to assess the efficiency of a new ex vivo confocal microscope for the per-operative assessment of surgical margins. Methods: We analyzed 16 biopsies and 93 surgical specimens of basal cell and squamous cell carcinomas by ex vivo CLSM using Histolog® Scanner V2. Surgical specimens included fusiform excisions, slow-Mohs peripheral and deep compartments, and Mohs excisions. The time required from surgical excision to image analysis was recorded and the quality of the images obtained for each specimen assessed. The presence or absence of tumour was estimated based on ex vivo CLSM images and compared with conventional H&E-stained sections from paraffin-embedded or frozen (Mohs) specimens. Results: Mean time for specimen processing using Histolog Scanner was 5.1 ± 3.4 min. We obtained 89% of high quality images. Mean time for confocal image analysis was 1 ± 0.76 min. The diagnostic sensitivity and specificity for ex vivo CLSM compared to classical H&E procedures were respectively 93% and 100% when performed on tumour biopsies. The overall sensitivity and specificity for ex vivo CLSM for margin assessment compared to classical H&E procedures were respectively 61.5% and 95%, with variations depending on the type of tumour or surgical specimen analyzed. In particular, we obtained 80% sensitivity and 100% specificity for the assessment of BCC surgical margins. Conclusion: Our data suggest that ex vivo CLSM using Histolog® Scanner V2 could be a valid help for surgeons for a fast and accurate per-operative margin analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.