Evidence map›Paper›PMID 35677457›Full record

ArticleData in brief2022

Labeled quantitative proteomics dataset of optogenetics induced axon regeneration in mice.

Faith Christine Harvey, Ximena Mendoza, Yuan Liu, Richard K Lee, Sanjoy K Bhattacharya

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Data in brief, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Faith Christine HarveyBascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Ximena MendozaMiami Integrative Metabolomics Research Center, Miami, FL 33136, USA.
Yuan LiuBascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Richard K LeeBascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
Sanjoy K BhattacharyaBascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
University of Miami · US

Funding

Shared Equipment ModuleP30EY014801 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI Victor L Perez · 2004 to 2026
$12.2M
Novel Targets to Promote RGC Axon Regeneration: Insights from Unique RGC CohortsU01EY027257 · NEI · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI BHATTACHARYA, SANJOY K, LEMMON, VANCE P · 2016 to 2018
$2.0M
NEI NIH HHS P30 EY014801NEI NIH HHS U01 EY027257
6 · The paper itself

Abstract

This labeled quantitative proteomics dataset was collected from a transgenic channel rhodopsin mouse model (Chr2) subjected to light stimulation after traumatic optic nerve crush (ONC). Protein extraction was performed by careful mincing of the tissue in extraction buffer (TEAB, NaCl and SDS). Protein amounts were normalized across samples using dot blot densitometry and ImageJ software. Samples were labeled for quantification using a modified TMTpro™ 16plex Label Reagent Set (Thermo Scientific™) after performing an overnight trypsin digestion. Untargeted liquid chromatography-mass spectrometry was performed on an Easy-nLC 1000 liquid chromatograph coupled to a Q Exactive mass spectrometer (LC-MS/MS). Data analysis was performed using Proteome Discoverer™ 2.5 (Thermo Scientific™). This data has been deposited to the ProteomeXchange (PX) and is available through PRIDE with the identifier PXD032788.

Indexed as

Axon regenerationOptogenetic stimulationQuantitative proteomeTMT-labeling

Identifiers

PMID35677457
PMCPMC9167879
OpenAlexW4281250413

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.