Evidence map›Paper›PMID 35674949›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Downregulation of Essential Genes in the Fungal Pathogen Candida auris.

Kali R Iyer, Sang Hu Kim, Nicole Robbins, Leah E Cowen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Defining thebioRxiv : the preprint server for biology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kali R Iyer *Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Sang Hu Kim *Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Nicole RobbinsDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Leah E CowenDepartment of Molecular Genetics, University of Toronto, Toronto, ON, Canada. leah.cowen@utoronto.ca.
University of Toronto · CA

Funding

Systematic Analysis of Morphogenesis, Commensalism, and Virulence in a Leading Human Fungal PathogenR01AI127375 · NIAID · UNIVERSITY OF TORONTO · PI LEAH Elizabeth Cowen · 2017 to 2026
$5.3M
Targeting Hsp90 in cryptococcal fungal pathogenesisR01AI120958 · NIAID · UNIVERSITY OF TORONTO · PI BROWN, LAUREN ELAINE, COWEN, LEAH ELIZABETH · 2016 to 2019
$3.2M
NIAID NIH HHS R01 AI120958NIAID NIH HHS R01 AI127375
6 · The paper itself

Abstract

The recent global emergence of the fungal pathogen Candida auris has caused significant concern given that this pathogen often exhibits resistance to multiple antifungal drug classes. In order to effectively combat C. auris infections, there is a dire need to expand our current antifungal arsenal. Essential proteins often serve as targets for antimicrobial compounds, and thus being able to study essential genes in a pathogen of interest is a critical first step in drug development. To identify and characterize essential genes in microorganisms, researchers must be able to manipulate microbial genomes using a variety of molecular biology techniques. Given the haploid genome of C. auris, genetic alterations have largely been achieved by gene deletion through homologous recombination using a drug resistance marker. However, this approach is not feasible to study essential gene function. Here, we describe a method for the study of essential genes using a tetracycline-repressible promoter replacement system, which can be used to genetically repress essential genes in C. auris and, thus, study their function. This method provides a powerful approach to assess and characterize essential gene function in an emerging fungal pathogen.

Indexed as

Candida aurisGenes, EssentialAntifungal AgentsDown-RegulationDrug Resistance, FungalMicrobial Sensitivity TestsAntifungal AgentsCandida aurisElectroporationEssential genesGene repressionHsp90Transformation

Identifiers

PMID35674949
PMCPMC11016342
OpenAlexW4285259964

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.