Evidence map›Paper›PMID 35674891›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Throughput Analysis to Identify Activators of Notch Signaling.

Rachael Guenter, Jacob Eide, Herbert Chen, J Bart Rose, Renata Jaskula-Sztul

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Rachael GuenterDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Jacob EideDepartment of Otolaryngology - Head and Neck Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Herbert ChenDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
J Bart RoseDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Renata Jaskula-SztulDepartment of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA. rjsztul@uabmc.edu.
University of Alabama at Birmingham · USNorthwestern University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Notch pathway regulates many cellular functions in a context-dependent manner. Depending on the cell type, either the activation or inhibition of Notch signaling can influence many processes such as cellular proliferation, specification, differentiation, and survival. The activation of Notch signaling has been shown to have therapeutic advantages in some cancers, thus having a method to identify Notch-activating compounds is needed. In this chapter we outline a method for high-throughput analysis of potential Notch pathway activators in a pancreatic neuroendocrine tumor cell line as an example. We also include the steps for subsequent validation of results and preclinical testing.

Indexed as

NeoplasmsReceptors, NotchCell ProliferationHumansSignal TransductionReceptors, NotchCancer therapeuticsHigh-throughput analysisLuciferase reporter plasmidNotch activationNotch pathwayNotch signaling

Identifiers

PMID35674891
OpenAlexW4285135324

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.