ArticleApplied microbiology and biotechnology2022
Identification of novel molecular targets for Weissella species-specific real-time PCR based on pangenome analysis.
Article in Applied microbiology and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 11 citations in OpenAlex.
- Development and preliminary evaluation of real-time PCR assays for six lactic acid bacteria.Scientific reports · 2026Article
- A Lateral Flow-Recombinase Polymerase Amplification Method forJournal of fungi (Basel, Switzerland) · 2024Article
- Differentiation betweenInternational journal of molecular sciences · 2023Article
- TheFrontiers in microbiology · 2023Review
- Identification ofFrontiers in microbiology · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Some Weissella species are used in probiotic products because of their beneficial effects in humans, whereas some species are considered as opportunistic pathogens that cause infections in humans. Therefore, an accurate and rapid identification of Weissella species is essential to control pathogenic Weissella species or isolate new functional strains with probiotic effects from their habitat. The objective of our study was to extract novel molecular targets using pangenome analysis for the identification of major Weissella species present in food. With 50 genomes representing 11 Weissella species, novel molecular targets were mined based on their 100% presence in the respective strains of the target species and absence in the strains of non-target bacteria. Primers based on molecular targets showed positive results for the corresponding species, whereas 79 non-target strains showed negative results. Standard curves revealed good linearity in the range of 10
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