ArticleScientific reports2022
5-Azacytidine increases tanshinone production in Salvia miltiorrhiza hairy roots through epigenetic modulation.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 23 citations in OpenAlex.
- Identification of DNA Methyltransferase/Demethylase Genes and 5-Azacytidine's Impact on β-Elemene and Methylation inBiology · 2025Article
- Molecular mechanisms and biological functions of active DNA demethylation in plants.Epigenetics & chromatin · 2025Review
- Characterization and differential expression of DNA methyltransferase and demethylase genes in response to abiotic stress in Isodon rubescens.BMC plant biology · 2025Article
- In vitro synthetic polyploidization and enhancement of anticancer compounds in Catharanthus Roseus (L.) G. Don important cultivars.Scientific reports · 2025Article
- DNA methylation valley as a distinguishing feature occurs in root-specific expressed nicotine-related genes inFrontiers in plant science · 2025Article
- Comparative transcriptome and metabolite profiling reveal diverse pattern of CYP-TS gene expression during corosolic acid biosynthesis in Lagerstroemia speciosa (L.) Pers.Plant cell reports · 2024Article
- The Smi-miR858a-Horticulture research · 2024Article
- Identification of the Effects of 5-Azacytidine on Porcine Circovirus Type 2 Replication in Porcine Kidney Cells.Veterinary sciences · 2024Article
- The Sink-Source Relationship in Cucumber (Plants (Basel, Switzerland) · 2023Article
- Characteristics ofHorticulture research · 2023Article
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
Recent studies have indicated strong connections between epigenetic modulation and secondary metabolites in plants. It is vital to understand the roles of epigenetics in the production of secondary metabolites. In this study, the inhibitor of DNA methylation 5-azacytidine (5-Az) was used on the hairy roots of the medicinal plant Salvia miltiorrhiza to investigate its effect on secondary metabolite production, gene expression, methylation levels in genomic DNA and promoter regions. Our results showed that the contents of tanshinones in S. miltiorrhiza hairy roots increased by 1.5-5 times, and some genes in the biosynthesis pathway showed an upward trend. According to our NGS analysis, the methylation pattern in the promotor of the gene encoding copalyl diphosphate synthase (CPS) was altered, and 51 out of 145 cytosines were demethylated during 5-Az treatment. A total of 36 putative transcription factors (TFs) binding cites were identified in these demethylation sites. Among these TFs binding cites, cis-regulatory elements for the binding of NF-Y and MYB were frequently found in our results. This is the first report to demonstrate a possible mechanism of DNA methylation participating in tanshinone biosynthesis in S. miltiorrhiza hairy roots by modulating the CPS promoter and TFs binding sites.
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