ArticleMolecular biology and evolution2022
Recurrent Plant-Specific Duplications of KNL2 and Its Conserved Function as a Kinetochore Assembly Factor.
Article in Molecular biology and evolution, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 20 citations in OpenAlex.
- The meiotic achilles' heel: vulnerability and resilience under environmental stress.Protoplasma · 2026Review
- Structural basis of βKNL2 centromeric targeting mechanism and its role in plant-specific kinetochore assembly.Nucleic acids research · 2026Article
- Kinetochore mutations and histone phosphorylation pattern changes accompany holo- and macro-monocentromere evolution.Nature communications · 2025Article
- Exploring potential strategies for haploid induction based on double fertilization in plants.Plant biotechnology journal · 2025Review
- Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.The Plant cell · 2025Article
- The kinesin motor POS3 and the microtubule polymerase MOR1 coordinate chromosome congression during mitosis in Arabidopsis.The Plant cell · 2025Article
- Centromeric localization of αKNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions.Nucleic acids research · 2025Article
- Plant kinetochore complex: composition, function, and regulation.Frontiers in plant science · 2024Review
- Exploring Plant Meiosis: Insights from the Kinetochore Perspective.Current issues in molecular biology · 2023Review
- Disruption of the standard kinetochore in holocentricProceedings of the National Academy of Sciences of the United States of America · 2023Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
KINETOCHORE NULL2 (KNL2) plays key role in the recognition of centromeres and new CENH3 deposition. To gain insight into the origin and diversification of the KNL2 gene, we reconstructed its evolutionary history in the plant kingdom. Our results indicate that the KNL2 gene in plants underwent three independent ancient duplications in ferns, grasses and eudicots. Additionally, we demonstrated that previously unclassified KNL2 genes could be divided into two clades αKNL2 and βKNL2 in eudicots and γKNL2 and δKNL2 in grasses, respectively. KNL2s of all clades encode the conserved SANTA domain, but only the αKNL2 and γKNL2 groups additionally encode the CENPC-k motif. In the more numerous eudicot sequences, signatures of positive selection were found in both αKNL2 and βKNL2 clades, suggesting recent or ongoing adaptation. The confirmed centromeric localization of βKNL2 and mutant analysis suggests that it participates in loading of new CENH3, similarly to αKNL2. A high rate of seed abortion was found in heterozygous βKNL2 plants and the germinated homozygous mutants did not develop beyond the seedling stage. Taken together, our study provides a new understanding of the evolutionary diversification of the plant kinetochore assembly gene KNL2, and suggests that the plant-specific duplicated KNL2 genes are involved in centromere and/or kinetochore assembly for preserving genome stability.
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