Evidence map›Paper›PMID 35670763›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2022

IL-10 promotes endothelial progenitor cell infiltration and wound healing via STAT3.

Walker D Short, Emily Steen, Aditya Kaul, Xinyi Wang, Oluyinka O Olutoye, Hima V Vangapandu, Natalie Templeman, Alexander J Blum, Chad M Moles, Daria A Narmoneva and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
6.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 78 citations in OpenAlex.

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  12. Endothelial Progenitor Cells: Disease Markers and Potential Therapy in Kidney Disease.Journal of the American Society of Nephrology : JASN · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 1 country.

Walker D ShortDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Emily SteenDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Aditya KaulDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Xinyi WangDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Oluyinka O OlutoyeDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Hima V VangapanduDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Natalie TemplemanDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Alexander J BlumDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Chad M MolesDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Daria A NarmonevaBiomedical Engineering, Department of Biomedical, Chemical and Environmental Engineering, College of Engineering and Applied Sciences, University of Cincinnati, Cincinnati, Ohio, USA.
Timothy M CrombleholmeDivision of Pediatric General Thoracic and Fetal Surgery, Connecticut Children's Hospital, University of Connecticut School of Medicine, Farmington, Connecticut, USA.
Manish J ButteDivision of Immunology, Allergy, and Rheumatology, Departments of Pediatrics and Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA.
Paul L BollykyDivision of Infectious Diseases, Department of Medicine, Stanford University School of Medicine, Stanford, California, USA.
Sundeep G KeswaniDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.
Swathi BalajiDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital and Baylor College of Medicine, Houston, Texas, USA.ORCID https://orcid.org/0000-0001-8875-8008
Baylor College of Medicine · USStanford University · USUniversity of California, Los Angeles · USUniversity of Cincinnati · USUniversity of Connecticut · US

Funding

Novel Mechanisms of Regenerative Wound HealingR01GM111808 · NIGMS · CINCINNATI CHILDRENS HOSP MED CTR · PI Sundeep G Keswani · 2014 to 2026
$3.6M
NIGMS NIH HHS R01 GM111808
6 · The paper itself

Abstract

Endothelial progenitor cells (EPCs) contribute to de novo angiogenesis, tissue regeneration, and remodeling. Interleukin 10 (IL-10), an anti-inflammatory cytokine that primarily signals via STAT3, has been shown to drive EPC recruitment to injured tissues. Our previous work demonstrated that overexpression of IL-10 in dermal wounds promotes regenerative tissue repair via STAT3-dependent regulation of fibroblast-specific hyaluronan synthesis. However, IL-10's role and specific mode of action on EPC recruitment, particularly in dermal wound healing and neovascularization in both normal and diabetic wounds, remain to be defined. Therefore, inducible skin-specific STAT3 knockdown mice were studied to determine IL-10's impact on EPCs, dermal wound neovascularization and healing, and whether it is STAT3-dependent. We show that IL-10 overexpression significantly elevated EPC counts in the granulating wound bed, which was associated with robust capillary lumen density and enhanced re-epithelialization of both control and diabetic (db/db) wounds at day 7. We noted increased VEGF and high C-X-C motif chemokine 12 (CXCL12) levels in wounds and a favorable CXCL12 gradient at day 3 that may support EPC mobilization and infiltration from bone marrow to wounds, an effect that was abrogated in STAT3 knockdown wounds. These findings were supported in vitro. IL-10 promoted VEGF and CXCL12 synthesis in primary murine dermal fibroblasts, with blunted VEGF expression upon blocking CXCL12 in the media by antibody binding. IL-10-conditioned fibroblast media also significantly promoted endothelial sprouting and network formation. In conclusion, these studies demonstrate that overexpression of IL-10 in dermal wounds recruits EPCs and leads to increased vascular structures and faster re-epithelialization.

Indexed as

Diabetes MellitusEndothelial Progenitor CellsAnimalsCulture Media, ConditionedInterleukin-10MiceNeovascularization, PhysiologicSTAT3 Transcription FactorVascular Endothelial Growth Factor AWound HealingCulture Media, ConditionedIL10 protein, mouseInterleukin-10Stat3 protein, mouseSTAT3 Transcription FactorVascular Endothelial Growth Factor Aangiogenesisdiabetesendothelial progenitor cellsIL-10VEGFwound healing

Identifiers

PMID35670763
PMCPMC9796147
OpenAlexW4281770536

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.