Evidence map›Paper›PMID 35670588›Full record

ArticleJournal of bacteriology2022

Genome-Wide Analysis and Characterization of the Riemerella anatipestifer Putative T9SS Secretory Proteins with a Conserved C-Terminal Domain.

Zongchao Chen, Pengfei Niu, Xiaomei Ren, Wenlong Han, Ruyu Shen, Min Zhu, Yang Yu, Shengqing Yu

Open access · greenAbstract read
In one paragraph

Article in Journal of bacteriology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Advances in the type IX secretion system: an exploration focusing onCurrent research in microbial sciences · 2025
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Zongchao ChenShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Pengfei NiuShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Xiaomei RenShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Wenlong HanShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Ruyu ShenShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Min ZhuShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.
Yang YuJiangsu Agri-animal Husbandry Vocational College, Taizhou, Jiangsu, China.
Shengqing YuShanghai Veterinary Research Institutegrid.464410.3, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, China.ORCID 0000-0003-1586-4277
Shanghai Veterinary Research Institute · CNJiangsu Agri-animal Husbandry Vocational College · CN

Funding

Co-innovation of Science and Technology Innovation Project in Chinese Academy of Agricultural Sciences CAAS-XTCX2016011-04-8School-level Scientific Research Project of Jiangsu Agri-animal Husbandry Vocational College NSF2022CB17Shanghai Key Science and Technology Innovation Action Plan 19391902800
6 · The paper itself

Abstract

Riemerella anatipestifer is a major pathogenic agent of duck septicemic and exudative diseases. Recent studies have shown that the R. anatipestifer type IX secretion system (T9SS) acts as a crucial virulence factor. We previously identified two T9SS component proteins, GldK and GldM, and one T9SS effector metallophosphoesterase, which play important roles in bacterial virulence. In this study, 19 T9SS-secreted proteins that contained a conserved T9SS C-terminal domain (CTD) were predicted in R. anatipestifer strain Yb2 by searching for CTD-encoding sequences in the whole genome. The proteins were confirmed with a liquid chromatography-tandem mass spectrometry analysis of the bacterial culture supernatant. Nine of them were reported in our previous study. We generated recombinant proteins and mouse antisera for the 19 predicted proteins to confirm their expression in the bacterial culture supernatant and in bacterial cells. Western blotting indicated that the levels of 14 proteins were significantly reduced in the T9SS mutant Yb2Δ

Indexed as

Poultry DiseasesRiemerellaAnimalsBacterial ProteinsDucksPeptide HydrolasesVirulence FactorsBacterial ProteinsPeptide HydrolasesVirulence Factorsbacterial virulenceprotease activityRiemerella anatipestifersecretory proteinT9SS

Identifiers

PMID35670588
PMCPMC9295540
OpenAlexW4281615687

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.