Evidence map›Paper›PMID 35670200›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2022

Block Length-Dependent Protein Fouling on Poly(2-oxazoline)-Based Polymersomes: Influence on Macrophage Association and Circulation Behavior.

Adrian Najer, Alexis Belessiotis-Richards, Hyemin Kim, Catherine Saunders, Federico Fenaroli, Christopher Adrianus, Junyi Che, Renée L Tonkin, Håkon Høgset, Samuel Lörcher and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 4 countries.

Adrian NajerDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0003-4868-9364
Alexis Belessiotis-RichardsDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0001-6838-5961
Hyemin KimDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0001-8534-3886
Catherine SaundersDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Federico FenaroliDepartment of Biosciences, University of Oslo, Blindernveien 31, Oslo, 0371, Norway.ORCID 0000-0002-9060-8786
Christopher AdrianusDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0002-3468-9665
Junyi CheDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Renée L TonkinDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Håkon HøgsetDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.
Samuel LörcherDepartment of Chemistry, University of Basel, Mattenstrasse 24a, BPR 1096, Basel, 4058, Switzerland.
Matthew PennaSchool of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia.
Stuart G HigginsDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0002-4653-5364
Wolfgang MeierDepartment of Chemistry, University of Basel, Mattenstrasse 24a, BPR 1096, Basel, 4058, Switzerland.
Irene YarovskySchool of Engineering, RMIT University, Melbourne, Victoria, 3001, Australia.ORCID 0000-0002-4033-5150
Molly M StevensDepartment of Materials, Department of Bioengineering, and Institute of Biomedical Engineering, Imperial College London, London, SW7 2AZ, UK.ORCID 0000-0002-1146-7090
Imperial College London · GBRMIT University · AUUniversity of Basel · CHUniversity of Oslo · NO

Funding

Cancer Research UK 30035Cancer Research UK C71717/A30035Medical Research Council MR/R015651/1Swiss National Science Foundation P2BSP2_168751Wellcome Trust 209121Wellcome Trust 209121/Z/17/ZWellcome Trust 209121_Z_17_Z
6 · The paper itself

Abstract

Polymersomes are vesicular structures self-assembled from amphiphilic block copolymers and are considered an alternative to liposomes for applications in drug delivery, immunotherapy, biosensing, and as nanoreactors and artificial organelles. However, the limited availability of systematic stability, protein fouling (protein corona formation), and blood circulation studies hampers their clinical translation. Poly(2-oxazoline)s (POx) are valuable antifouling hydrophilic polymers that can replace the current gold-standard, poly(ethylene glycol) (PEG), yet investigations of POx functionality on nanoparticles are relatively sparse. Herein, a systematic study is reported of the structural, dynamic and antifouling properties of polymersomes made of poly(2-methyl-2-oxazoline)-block-poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PMOXA-b-PDMS-b-PMOXA). The study relates in vitro antifouling performance of the polymersomes to atomistic molecular dynamics simulations of polymersome membrane hydration behavior. These observations support the experimentally demonstrated benefit of maximizing the length of PMOXA (degree of polymerization (DP) > 6) while keeping PDMS at a minimal length that still provides sufficient membrane stability (DP > 19). In vitro macrophage association and in vivo blood circulation evaluation of polymersomes in zebrafish embryos corroborate these findings. They further suggest that single copolymer presentation on polymersomes is outperformed by blends of varied copolymer lengths. This study helps to rationalize design rules for stable and low-fouling polymersomes for future medical applications.

Indexed as

Drug Delivery SystemsZebrafishAnimalsHydrophobic and Hydrophilic InteractionsMacrophagesOxazolesOxazolespoly(2-oxazoline)atomistic simulationsnanoparticlesprotein coronaprotein foulingzebrafish embryos

Identifiers

PMID35670200
PMCPMC7615485
OpenAlexW4281736180

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.