ArticleHorticulture research2022
Engineering high levels of saffron apocarotenoids in tomato.
Article in Horticulture research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 58 citations in OpenAlex.
- Fruit-Specific Promoters in Plants: Advances, Regulatory Mechanisms and Applications in Plant Biotechnology.Plants (Basel, Switzerland) · 2026Review
- Bioconversion of carotenoids into high-value crocins using a marine sponge carotenoid cleavage dioxygenase.The New phytologist · 2026Article
- Comparative metabolomics identifies enhanced ursane-type triterpenoids and antioxidant capacity inFrontiers in plant science · 2026Article
- Heterologous Biosynthesis of Crocin I inInternational journal of molecular sciences · 2025Article
- Novel plant cell suspension platforms for saffron apocarotenoid production and its impact on carotenoid and volatile profiles.Plant biotechnology journal · 2025Article
- Natural CCD2 Variants and RNA Interference for Boosting Crocin Biosynthesis in Tomato.Biology · 2025Article
- CsCCD2L is Involved in the Response to Light Stress by Regulating Zeaxanthin Content in Saffron (Crocus sativus L.).Plant foods for human nutrition (Dordrecht, Netherlands) · 2025Article
- Chromatic symphony of fleshy fruits: functions, biosynthesis and metabolic engineering of bioactive compounds.Molecular horticulture · 2025Review
- Water-soluble carotenoid: focused on natural carotenoid crocin.Food science and biotechnology · 2025Review
- Development of a UHPLC-MS method to avoid the in-source dissociation interference in characterization of crocins from Buddlejae flos and its dyeing yellow rice.Frontiers in plant science · 2025Article
- Optimizing a tomato crocin biofactory by fine-tuning plant architecture.Frontiers in plant science · 2025Article
- Phytoene and Phytoene-Rich Microalgae Extracts Extend Lifespan inAntioxidants (Basel, Switzerland) · 2024Article
- Neuroprotective properties of exosomes and chitosan nanoparticles of Tomafran, a bioengineered tomato enriched in crocins.Natural products and bioprospecting · 2024Article
- Research progress on the pharmacological activity, biosynthetic pathways, and biosynthesis of crocins.Beilstein journal of organic chemistry · 2024Review
- Research Progress in Heterologous Crocin Production.Marine drugs · 2023Review
- Synthesis of Crocin I and Crocin II by Multigene Stacking inInternational journal of molecular sciences · 2023Article
- Towards carotenoid biofortification in wheat: identification of XAT-7A1, a multicopy tandem gene responsible for carotenoid esterification in durum wheat.BMC plant biology · 2023Article
- Production of Saffron Apocarotenoids inMetabolites · 2023Article
- Effects of Vegetal Extracts and Metabolites against Oxidative Stress and Associated Diseases: Studies inACS omega · 2023Review
- G2-LIKE CAROTENOID REGULATOR (SlGCR) is a positive regulator of lutein biosynthesis in tomato.aBIOTECH · 2022Article
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crocins and picrocrocin are high-value hydrophilic pigments produced in saffron and used commercially in the food and pharmaceutical industries. These apocarotenoids are derived from the oxidative cleavage of zeaxanthin by specific carotenoid cleavage dioxygenases. The pathway for crocins and picrocrocin biosynthesis was introduced into tomato using fruit specific and constitutive promoters and resulted in 14.48 mg/g of crocins and 2.92 mg/g of picrocrocin in the tomato DW, without compromising plant growth. The strategy involved expression of CsCCD2L to produce crocetin dialdehyde and 2,6,6-trimethyl-4-hydroxy-1-carboxaldehyde-1-cyclohexene, and of glycosyltransferases UGT709G1 and CsUGT2 for picrocrocin and crocins production, respectively. Metabolic analyses of the engineered fruits revealed picrocrocin and crocetin-(β-D-gentiobiosyl)-(β-D-glucosyl)-ester, as the predominant crocin molecule, as well as safranal, at the expense of the usual tomato carotenoids. The results showed the highest crocins content ever obtained by metabolic engineering in heterologous systems. In addition, the engineered tomatoes showed higher antioxidant capacity and were able to protect against neurological disorders in a
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.