Evidence map›Paper›PMID 35668362›Full record

ArticleBMC microbiology2022

Whole-genome-sequence-based characterization of an NDM-5-producing uropathogenic Escherichia coli EC1390.

Tran Thi Dieu Thuy, Hsu-Feng Lu, Pei-Yun Kuo, Wei-Hung Lin, Tzu-Ping Lin, Yi-Tzu Lee, Tran Thi Thuy Duong, Ming-Cheng Wang, Yi-Hong Lee, Li-Li Wen and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  5. Prevalence and molecular characteristics of polymyxin-resistantFrontiers in cellular and infection microbiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 1 country.

Tran Thi Dieu Thuy *Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan.
Hsu-Feng Lu *Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
Pei-Yun Kuo *Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan.
Wei-Hung LinDepartment of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.
Tzu-Ping LinDepartment of Urology, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
Yi-Tzu LeeDepartment of Emergency Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.
Tran Thi Thuy DuongInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan.
Ming-Cheng WangDepartment of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.
Yi-Hong LeeInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan.
Li-Li WenDepartment of Clinical Laboratory, En Chu Kong Hospital, New Taipei City, Taiwan.
Yu-Chen ChenGenomics Research Center, Academia Sinica, Taipei, Taiwan.
Cheng-Yen KaoInstitute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, No.155, Sec.2, Linong Street, Taipei, 112, Taiwan. kaocy@nycu.edu.tw.
National Yang Ming Chiao Tung University · TWNational Cheng Kung University Hospital · TWChina Medical University Hospital · TWEn Chu Kong Hospital · TWGenomics Research Center, Academia Sinica · TWTaipei Veterans General Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundUrinary tract infection (UTI) is one of the most common outpatient bacterial infections. In this study, we isolated and characterized an extensively-drug resistant (XDR) NDM-5-producing Escherichia coli EC1390 from a UTI patient by using whole-genome sequencing (WGS) in combination with phenotypic assays.

methodsAntimicrobial susceptibility to 23 drugs was determined by disk diffusion method. The genome sequence of EC1390 was determined by Nanopore MinION MK1C platform. Conjugation assays were performed to test the transferability of EC1390 plasmids to E. coli recipient C600. Phenotypic assays, including growth curve, biofilm formation, iron acquisition ability, and cell adhesion, were performed to characterize the function of EC1390 plasmids.

resultsOur results showed that EC1390 was only susceptible to tigecycline and colistin, and thus was classified as XDR E. coli. A de novo genome assembly was generated using Nanopore 73,050 reads with an N

conclusionsIn summary, we characterized the genome of XDR-E. coli EC1390 and identified two plasmids contributing to the antimicrobial resistance, growth of bacteria in a nutrition-limited medium, biofilm formation, and cell adhesion.

Indexed as

Escherichia coli InfectionsUrinary Tract InfectionsUropathogenic Escherichia coliAnti-Bacterial Agentsbeta-LactamasesHumansIronMicrobial Sensitivity TestsPhylogenyPlasmidsAnti-Bacterial Agentsbeta-LactamasesIronbla NDM-5Extensively-drug resistantUrinary tract infectionVirulenceWhole-genome sequencing

Identifiers

PMID35668362
PMCPMC9172118
OpenAlexW4281613547

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.