ArticleNature microbiology2022
Bacillus subtilis biofilm matrix components target seed oil bodies to promote growth and anti-fungal resistance in melon.
Article in Nature microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 88 citations in OpenAlex.
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- A chemical bactericide dioctyldiethylenetriamine (Xinjunan) exerts a non-lethal effect by inhibiting RpfG activity to regulate the quorum sensing system.PLoS pathogens · 2026Article
- Early seed priming with closely relatedHorticulture research · 2026Article
- What do we know about the seed microbiome?Microbiome · 2026Review
- Novel approaches for understanding and improving the effectiveness of seed biopriming.Scientific reports · 2026Article
- Long-term organic farming shapes the avocado rhizosphere microbiota through the enrichment of drought-tolerant Bacillus spp.NPJ biofilms and microbiomes · 2026Article
- Biofilms of Bacillus subtilis as biostimulants for plant resilience to drought and flooding.Scientific reports · 2026Article
- Biofilms: from the cradle of life to life support.NPJ biofilms and microbiomes · 2026Review
- Surfactin-Bacillaene Copathway Engineering Strategy Boosts Fengycin Production and Antifungal Activity inMicroorganisms · 2026Article
- Comparative analysis of the seed microbiome in four major oilseed crops (rapeseed, sunflower, soybean, sesame) reveals host-specific assembly and potential application of seed core microbes.Frontiers in plant science · 2026Article
- Synthetic Protein-Assisted Co-Assembly of Zeolitic Imidazolate Framework-8 andMolecules (Basel, Switzerland) · 2025Article
- The seed microbiota from an application perspective: an underexplored frontier in plant-microbe interactions.Crop health · 2025Review
- Elongation factor Tu promotes the onset of periodontitis through mediating bacteria adhesion.NPJ biofilms and microbiomes · 2025Article
- Differential responses of root and leaf-associated microbiota to continuous monocultures.Environmental microbiome · 2025Article
- Review
- Diversification of Lipopeptide Analogues Drives Versatility in Biological Activities.Journal of agricultural and food chemistry · 2025Article
- Hypoxia induces phenotypic and metabolic shifts in endophytic Flavobacterium sp. 98.The ISME journal · 2025Article
- Statistical analysis of feature-based molecular networking results from non-targeted metabolomics data.Nature protocols · 2025Article
- Biotechnological advances in plant growth-promoting rhizobacteria for sustainable agriculture.World journal of microbiology & biotechnology · 2024Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 4 countries.
Funding
Abstract
Beneficial microorganisms are used to stimulate the germination of seeds; however, their growth-promoting mechanisms remain largely unexplored. Bacillus subtilis is commonly found in association with different plant organs, providing protection against pathogens or stimulating plant growth. We report that application of B. subtilis to melon seeds results in genetic and physiological responses in seeds that alter the metabolic and developmental status in 5-d and 1-month-old plants upon germination. We analysed mutants in different components of the extracellular matrix of B. subtilis biofilms in interaction with seeds and found cooperation in bacterial colonization of seed storage tissues and growth promotion. Combining confocal microscopy with fluorogenic probes, we found that two specific components of the extracellular matrix, amyloid protein TasA and fengycin, differentially increased the concentrations of reactive oxygen species inside seeds. Further, using electron and fluorescence microscopy and metabolomics, we showed that both TasA and fengycin targeted the oil bodies in the seed endosperm, resulting in specific changes in lipid metabolism and accumulation of glutathione-related molecules. In turn, this results in two different plant growth developmental programmes: TasA and fengycin stimulate the development of radicles, and fengycin alone stimulate the growth of adult plants and resistance in the phylloplane to the fungus Botrytis cinerea. Understanding mechanisms of bacterial growth promotion will enable the design of bespoke growth promotion strains.
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Registered trials
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