ArticlePLoS neglected tropical diseases2022
Comparison of the Serion IgM ELISA and Microscopic Agglutination Test for diagnosis of Leptospira spp. infections in sera from different geographical origins and estimation of Leptospira seroprevalence in the Wiwa indigenous population from Colombia.
Article in PLoS neglected tropical diseases, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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13 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Epidemiology of human and animal leptospirosis in Kenya: A systematic review and meta-analysis of disease occurrence, serogroup diversity and risk factors.PLoS neglected tropical diseases · 2024Pooled it
- A recombinant Loa22-based latex agglutination test and indirect ELISA for the sero-detection of leptospirosis in dogs and cattle.Archives of microbiology · 2026Article
- Subclinical Leptospiral Infections and Antibody Waning During Pregnancy: A Population-Based Cohort Study from Rural Sri Lanka.The American journal of tropical medicine and hygiene · 2026Article
- Advances in ELISA-based detection of equine leptospirosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Evaluation of a recombinant Loa22-gold nanoparticle based lateral flow assay for the serodiagnosis of leptospirosis in canine and bovine.Archives of microbiology · 2026Article
- Evolution of human leptospirosis in French Guiana, 2016-2022.PLoS neglected tropical diseases · 2025Article
- Review
- Evaluation of Serological Tests for Different Disease Stages of Leptospirosis Infection in Humans.Tropical medicine and infectious disease · 2024Article
- Seroprevalence and risk factors for Q fever and Rift Valley fever in pastoralists and their livestock in Afar, Ethiopia: A One Health approach.PLoS neglected tropical diseases · 2024Article
- Seroprevalence of Coxiella burnetii in an Indigenous Population from the Sierra Nevada De Santa Marta, Colombia.The American journal of tropical medicine and hygiene · 2024Article
- Molecular Diagnosis as an Alternative for Public Health Surveillance of Leptospirosis in Colombia.Microorganisms · 2023Review
- A clinical pilot study for the detection of sphingomyelinase in leptospirosis patient's urine at tertiary care hospital.Heliyon · 2023Article
- Enteric Bacteria and Parasites with Pathogenic Potential in Individuals of the Colombian Indigenous Tribe Kogui.Microorganisms · 2022Article
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Authors and funding
17 authors at 9 institutions in 6 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is among the most important zoonotic diseases in (sub-)tropical countries. The research objective was to evaluate the accuracy of the Serion IgM ELISA EST125M against the Microscopic Agglutination Test (MAT = imperfect reference test); to assess its ability to diagnose acute leptospirosis infections and to detect previous exposure to leptospires in an endemic setting. In addition, to estimate the overall Leptospira spp. seroprevalence in the Wiwa indigenous population in North-East Colombia. We analysed serum samples from confirmed leptospirosis patients from the Netherlands (N = 14), blood donor sera from Switzerland (N = 20), and sera from a cross-sectional study in Colombia (N = 321). All leptospirosis ELISA-positive, and a random of negative samples from Colombia were tested by the MAT for confirmation. The ELISA performed with a sensitivity of 100% (95% CI 77% - 100%) and a specificity of 100% (95% CI 83% - 100%) based on MAT confirmed Leptospira spp. positive and negative samples. In the cross-sectional study in Colombia, the ELISA performed with a sensitivity of 100% (95% CI 2-100%) and a specificity of 21% (95% CI 15-28%). Assuming a 5% Leptospira spp. seroprevalence in this population, the positive predictive value was 6% and the negative predictive value 100%. The Leptospira spp. seroprevalence in the Wiwas tested by the ELISA was 39%; however, by MAT only 0.3%. The ELISA is suitable to diagnose leptospirosis in acutely ill patients in Europe several days after onset of disease. For cross-sectional studies it is not recommended due to its low specificity. Despite the evidence of a high leptospirosis prevalence in other study areas and populations in Colombia, the Wiwa do not seem to be highly exposed to Leptospira spp.. Nevertheless, leptospirosis should be considered and tested in patients presenting with febrile illness.
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