ArticleDevelopment (Cambridge, England)2022
Generation of a homozygous mutant drug transporter (ABCB1) knockout line in the sea urchin Lytechinus pictus.
Article in Development (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Cryopreservation of sea urchin (Lytechinus pictus) embryos and development through metamorphosis.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- Cryopreservation and other preservation approaches for animal models workshop session I: Invertebrate models in biomedical research.Cryobiology · 2026Article
- Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026Article
- Automated, high-throughput in situ hybridization of sea urchin (Lytechinus pictus) embryos.Development (Cambridge, England) · 2025Article
- Characterization of cellular and molecular immune components of the painted white sea urchin Lytechinus pictus in response to bacterial infection.Immunology and cell biology · 2025Article
- Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus.Development (Cambridge, England) · 2024Article
- Localization and origins of juvenile skeletogenic cells in the sea urchin Lytechinuspictus.Developmental biology · 2024Article
- Conservation and contrast in cell states of echinoderm ovaries.Molecular reproduction and development · 2024Article
- Cold storage and cryopreservation methods for spermatozoa of the sea urchins Lytechinus pictus and Strongylocentrotus purpuratus.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Article
- The roles of ABCB1/P-glycoprotein drug transporters in regulating gut microbes and inflammation: insights from animal models, old and new.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Semi-automated, high-content imaging of drug transporter knockout sea urchin (Lytechinus pictus) embryos.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- Solute carrier (SLC) expression reveals skeletogenic cell diversity.Developmental biology · 2023Article
- Future research directions of the model marine tubeworm Hydroides elegans and synthesis of developmental staging of the complete life cycle.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- CRISPR/Cas9 knockin methodology for the sea urchin embryo.Molecular reproduction and development · 2023Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
Sea urchins are premier model organisms for the study of early development. However, the lengthy generation times of commonly used species have precluded application of stable genetic approaches. Here, we use the painted sea urchin Lytechinus pictus to address this limitation and to generate a homozygous mutant sea urchin line. L. pictus has one of the shortest generation times of any currently used sea urchin. We leveraged this advantage to generate a knockout mutant of the sea urchin homolog of the drug transporter ABCB1, a major player in xenobiotic disposition for all animals. Using CRISPR/Cas9, we generated large fragment deletions of ABCB1 and used these readily detected deletions to rapidly genotype and breed mutant animals to homozygosity in the F2 generation. The knockout larvae are produced according to expected Mendelian distribution, exhibit reduced xenobiotic efflux activity and can be grown to maturity. This study represents a major step towards more sophisticated genetic manipulation of the sea urchin and the establishment of reproducible sea urchin animal resources.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.