Single-Molecule FRET Studies of RNA Structural Rearrangements and RNA-RNA Interactions.

Ewelina M Małecka, Boyang Hua, Sarah A Woodson

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Mechanistic insights into RNA chaperoning by Ro60 and La autoantigens.Cell · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ewelina M MałeckaT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD, USA.

Boyang HuaDepartment of Molecular Biology and Genetics, Johns Hopkins Medical School, Baltimore, MD, USA.

Sarah A WoodsonT.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD, USA. swoodson@jhu.edu.

Funding

Assembly Mechanisms of RNA-Protein Complexes for Genetic ControlR35GM136351 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI SARAH A. WOODSON · 2020 to 2026

$4.4M

NIGMS NIH HHS R35 GM136351

6 · The paper itself

Abstract

RNA-guided regulation of gene expression is found in all cell types. In this mode of regulation, antisense interactions between the regulatory RNA and its target are typically facilitated by a protein partner. Single-molecule fluorescence microscopy is a powerful tool for dissecting the conformational states and intermediates that contribute to target recognition. This chapter describes protocols for studying target recognition by bacterial small RNAs and their chaperone Hfq on the single-molecule level, using a total internal reflection fluorescence microscope. The sections cover the design of suitable RNA substrates for sRNA-mRNA annealing reactions, preparation of internally labeled mRNA for detecting conformational changes in the target, and key steps of the data analysis. These protocols can be adapted to other RNA-binding proteins that chaperone RNA interactions.

Indexed as

Escherichia coli ProteinsRNA, Small UntranslatedFluorescence Resonance Energy TransferGene Expression Regulation, BacterialHost Factor 1 ProteinMolecular ChaperonesRNA, BacterialRNA, MessengerEscherichia coli ProteinsHost Factor 1 ProteinMolecular ChaperonesRNA, BacterialRNA, MessengerRNA, Small UntranslatedAntisense-RNA interactionsHfqRNA chaperoneSingle-molecule fluorescenceSmall RNATIRF

Identifiers

PMID35666451

DOI10.1007/978-1-0716-2421-0_16

PMCPMC10052914

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.