ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2022
Quantification of infectious Human mastadenovirus in environmental matrices using PMAxx-qPCR.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Refining microbial biomarker identification in rumen microbiome studies: a viability PCR-based approach.Applied and environmental microbiology · 2025Article
- Measurement of the Infection and Integrity of Monkeypox Virus: A New Method Using PMAxx-ddPCR.International journal of molecular sciences · 2025Article
- A reliable qPCR technique for detecting viable Xanthomonas arboricola pv. pruni cells.Applied microbiology and biotechnology · 2024Article
- Article
- A Novel RAA Combined Test Strip Method Based on Dual Gene Targets for PathogenicFoods (Basel, Switzerland) · 2023Article
- Quickly assessing disinfection effectiveness to control the spread of African swine fever virus.Applied microbiology and biotechnology · 2023Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular methodologies providing data on viral concentration and infectivity have been successfully used in environmental virology, supporting quantitative risk assessment studies. The present study aimed to assess human mastadenovirus (HAdV) intact particles using a derivative of propidium monoazide associated with qPCR (PMAxx-qPCR) in aquatic matrices. Initially, different concentrations of PMAxx were evaluated to establish an optimal protocol for treating different naturally contaminated matrices, using 10 min incubation in the dark at 200 rpm at room temperature and 15 min of photoactivation in the PMA-Lite™ LED photolysis device. There was no significant reduction in the quantification of infectious HAdV with increasing concentration of PMAxx used (20 μM, 50 μM, and 100 μM), except for sewage samples. In this matrix, a reduction of 5.01 log of genomic copies (GC)/L was observed from the concentration of 50 μM and revealed 100% HAdV particles with damaged capsids. On the other hand, the mean reduction of 0.51 log in stool samples using the same concentration mentioned above demonstrated 83% of damaged particles eliminated in the stool. Following, 50 μM PMAxx-qPCR protocol revealed a log reduction of 0.91, 0.67, and 1.05 in other samples of raw sewage, brackish, and seawater where HAdV concentration reached 1.47 × 10
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