Evidence map›Paper›PMID 35666382›Full record

ArticleApplied biochemistry and biotechnology2022

Data-Independent Acquisition-Based Mass Spectrometry (DIA-MS) for Quantitative Analysis of Human Intestinal Ischemia/Reperfusion.

Anzhong Huang, Wei Wu, Song Chen, Hanbing Hu, Jie Shen, Jingbo Qie, Ming Zhong, Lin Zhang

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anzhong Huang *Department of General Surgery, Jinshan Hospital, Fudan University, Shanghai, 201508, People's Republic of China.
Wei Wu *Department of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Song ChenDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Hanbing HuCenter of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China.
Jie ShenCenter of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China.
Jingbo QieInstitutes of Biomedical Sciences, Fudan University, Shanghai, China. jingboqie@fudan.edu.cn.
Ming ZhongDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China. zhong_ming@fudan.edu.cn.
Lin ZhangCenter of Emergency and Intensive Care Unit, Jinshan Hospital, Fudan University, Shanghai, China. linzhang0315@fudan.edu.cn.ORCID http://orcid.org/0000-0002-0950-7145

Funding

National Natural Science Foundation of China 81801943
6 · The paper itself

Abstract

Intestinal ischemia-reperfusion (II/R) injury is a complex pathologic process, which is of great significance to unravel the underlying mechanisms and pathophysiology. Our study represented a comprehensive proteomic analysis in the human intestine with ischemia-reperfusion injury. The proteomics analysis measured a total of 5,230 proteins, and 417 differently expressed proteins (DEPs) were identified between II/R and control samples. GO and KEGG analysis demonstrated that the 290 upregulated DEPs in II/R were significantly involved in immune-related biological process and tight junction, focal adhesion, and cAMP signaling pathway, whereas the 127 downregulated DEPs in II/R were enriched in lipid metabolic process and metabolic pathway. Furthermore, we screened out 20 hub proteins from the protein-protein interaction (PPI) network according to the degree of connectivity, and six clusters were identified. Combined with the result of KEGG analysis, 6 from the 20 hub proteins, ACTB, CAV1, FLNA, MYLK, ACTN1, and MYL9, were identified as the key proteins in the progress of II/R injury. According to the previous studies, FLNA and MYL9 were selected as the novel disease-related proteins for the first time. In conclusion, this study extended our understanding of the alteration in the human intestine during ischemia and reperfusion and highlighted the potential role of FLNA and MYL9 in the progress of II/R injury, which need to be further studied.

Indexed as

ProteomicsReperfusion InjuryHumansIntestinesMass SpectrometryReperfusionBioinformaticsData-independent acquisitionIntestinal ischemia/reperfusionMass spectrometryProteomics

Identifiers

PMID35666382

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.