ArticleScientific reports2022
Genome-wide specificity of plant genome editing by both CRISPR-Cas9 and TALEN.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 16 citations in OpenAlex.
- Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.BMC biotechnology · 2026Article
- The Importance of thePlants (Basel, Switzerland) · 2026Review
- Programmable genome engineering and gene modifications for plant biodesign.Plant communications · 2025Review
- Chromosome engineering points to the cis-acting mechanism of chromosome arm-specific telomere length setting and robustness of plant phenotype, chromatin structure and gene expression.The Plant journal : for cell and molecular biology · 2025Article
- A technical approach to global plant genome editing regulation.Nature biotechnology · 2024Article
- Can genome editing help transitioning to agroecology?iScience · 2024Review
- Genome-wide evaluation of gene editing outcomes using CRISPR/Cas9 in seed propagatedFrontiers in plant science · 2024Article
- Reproducibility of next-generation-sequencing-based analysis of a CRISPR/Cas9 genome edited oil seed rape.Food chemistry. Molecular sciences · 2023Article
- Improved prime editing allows for routine predictable gene editing in Physcomitrium patens.Journal of experimental botany · 2023Article
- Converting Hybrid Potato Breeding Science into Practice.Plants (Basel, Switzerland) · 2023Review
- BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize.The Plant cell · 2023Article
- High-throughput sgRNA testing reveals rules for Cas9 specificity and DNA repair in tomato cells.Frontiers in genome editing · 2023Article
- BabyBoom: 3-Dimensional Structure-Based Ligand and Protein Interaction Prediction by Molecular Docking.Biomolecules · 2022Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CRISPR and TALENs are efficient systems for gene editing in many organisms including plants. In many cases the CRISPR-Cas or TALEN modules are expressed in the plant cell only transiently. Theoretically, transient expression of the editing modules should limit unexpected effects compared to stable transformation. However, very few studies have measured the off-target and unpredicted effects of editing strategies on the plant genome, and none of them have compared these two major editing systems. We conducted, in Physcomitrium patens, a comprehensive genome-wide investigation of off-target mutations using either a CRISPR-Cas9 or a TALEN strategy. We observed a similar number of differences for the two editing strategies compared to control non-transfected plants, with an average of 8.25 SNVs and 19.5 InDels for the CRISPR-edited plants, and an average of 17.5 SNVs and 32 InDels for the TALEN-edited plants. Interestingly, a comparable number of SNVs and InDels could be detected in the PEG-treated control plants. This shows that except for the on-target modifications, the gene editing tools used in this study did not show a significant off-target activity nor unpredicted effects on the genome, and did not lead to transgene integration. The PEG treatment, a well-established biotechnological method, in itself, was the main source of mutations found in the edited plants.
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