ArticleFrontiers in plant science2022
Low-Input High-Molecular-Weight DNA Extraction for Long-Read Sequencing From Plants of Diverse Families.
Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
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- Uncovering the genome evolutionary dynamics of the Mediterranean endemic palm Chamaerops humilis.BMC plant biology · 2026Article
- Genome Assemblies of the Daisy Gorteria diffusa Elucidate the Molecular-Genomic Basis of Pollination by Sexual Deception.Genome biology and evolution · 2026Article
- Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification.Small methods · 2026Article
- Cookbook for plant genome sequences.BMC genomics · 2026Review
- Foliar fungal symbionts in sympatric yellow monkeyflowers along elevation gradients in the Sierra Nevada.bioRxiv : the preprint server for biology · 2026Article
- Ionic liquid-assisted seed genomic DNA extraction for advanced sequencing applications.Plant methods · 2025Article
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- A pangenome reveals LTR repeat dynamics as a major driver of genome evolution in Chenopodium.The plant genome · 2025Article
- Low-pass nanopore sequencing for measurement of global methylation levels in plants.BMC genomics · 2024Article
- Unlocking plant genetics with telomere-to-telomere genome assemblies.Nature genetics · 2024Review
- Genome of the early spider-orchid Ophrys sphegodes provides insights into sexual deception and pollinator adaptation.Nature communications · 2024Article
- A pan-genome of 69 Arabidopsis thaliana accessions reveals a conserved genome structure throughout the global species range.Nature genetics · 2024Article
- A DNA Extraction Method for Nondestructive Testing and Evaluation of Cotton Seeds (Gossypium L.).Biochemical genetics · 2024Article
- Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital.Annals of clinical microbiology and antimicrobials · 2024Article
- Article
- An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis.Plant communications · 2023Article
- Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds.Plants (Basel, Switzerland) · 2023Article
- Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-read DNA sequencing technologies require high molecular weight (HMW) DNA of adequate purity and integrity, which can be difficult to isolate from plant material. Plant leaves usually contain high levels of carbohydrates and secondary metabolites that can impact DNA purity, affecting downstream applications. Several protocols and kits are available for HMW DNA extraction, but they usually require a high amount of input material and often lead to substantial DNA fragmentation, making sequencing suboptimal in terms of read length and data yield. We here describe a protocol for plant HMW DNA extraction from low input material (0.1 g) which is easy to follow and quick (2.5 h). This method successfully enabled us to extract HMW from four species from different families (Orchidaceae, Poaceae, Brassicaceae, Asteraceae). In the case of recalcitrant species, we show that an additional purification step is sufficient to deliver a clean DNA sample. We demonstrate the suitability of our protocol for long-read sequencing on the Oxford Nanopore Technologies PromethION
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