Evidence map›Paper›PMID 35665018›Full record

ArticlebioRxiv : the preprint server for biology2022

The SARS-CoV-2 spike protein binds and modulates estrogen receptors.

Oscar Solis, Andrea R Beccari, Daniela Iaconis, Carmine Talarico, Camilo A Ruiz-Bedoya, Jerome C Nwachukwu, Annamaria Cimini, Vanessa Castelli, Riccardo Bertini, Monica Montopoli and 21 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

31 authors at 10 institutions in 3 countries.

Oscar SolisBiobehavioral Imaging and Molecular Neuropsychopharmacology Unit, National Institute on Drug Abuse Intramural Research Program, Baltimore, 21224, MD, USA.
Andrea R BeccariEXSCALATE, Dompé farmaceutici S.p.A, Napoli, Italy.
Daniela IaconisEXSCALATE, Dompé farmaceutici S.p.A, Napoli, Italy.
Carmine TalaricoEXSCALATE, Dompé farmaceutici S.p.A, Napoli, Italy.
Camilo A Ruiz-BedoyaCenter for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jerome C NwachukwuDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA.
Annamaria CiminiDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
Vanessa CastelliDepartment of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
Riccardo BertiniAtreius S.a.s., L'Aquila, Italy.
Monica MontopoliDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Veronica CocettaDepartment of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy.
Stefano BorocciDepartment for Innovation in Biological, Agro-Food and Forest Systems, DIBAF, University of Tuscia, Viterbo, Italy.
Ingrid G PrandiDepartment for Innovation in Biological, Agro-Food and Forest Systems, DIBAF, University of Tuscia, Viterbo, Italy.
Kelly FlavahanCenter for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Melissa BahrCenter for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Anna NapiorkowskiCenter for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Giovanni ChillemiDepartment for Innovation in Biological, Agro-Food and Forest Systems, DIBAF, University of Tuscia, Viterbo, Italy.
Masato OokaDivision of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville, MD, USA.
Xiaoping YangDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Shiliang ZhangNeuronal Networks Section, National Institute on Drug Abuse Intramural Research Program, Baltimore, 21224, MD, USA.
Menghang XiaDivision of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville, MD, USA.
Wei ZhengDivision of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville, MD, USA.
Jordi BonaventuraDepartament de Patologia i Terapèutica Experimental, Institut de Neurociències, Universitat de Barcelona, L'Hospitalet de Llobregat, Catalonia.
Martin G PomperDepartment of Radiology and Radiological Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Jody E HooperDepartment of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
Marisela MoralesNeuronal Networks Section, National Institute on Drug Abuse Intramural Research Program, Baltimore, 21224, MD, USA.
Avi Z RosenbergDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Kendall W NettlesDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA.
Sanjay K JainCenter for Infection and Inflammation Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Marcello AllegrettiDompé farmaceutici S.p.A, L'Aquila, Italy.
Michael MichaelidesBiobehavioral Imaging and Molecular Neuropsychopharmacology Unit, National Institute on Drug Abuse Intramural Research Program, Baltimore, 21224, MD, USA.
Johns Hopkins University · USDompé (Italy) · ITNational Institute on Drug Abuse · USNational Center for Advancing Translational Sciences · USUniversità degli Studi della Tuscia · ITScripps Research Institute · USUniversity of L'Aquila · ITUniversity of Padua · ITStanford University · USUniversitat de Barcelona · ES

Funding

Neuronal connectivity within the mesolimbic systemZIADA000511 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI MORALES, MARISELA · 2009 to 2025
$35.0M
Biobehavioral Imaging and Molecular Neuropsychopharmacology of Substance Abuse MechanismsZIADA000069 · NIDA · NATIONAL INSTITUTE ON DRUG ABUSE · PI MICHAELIDES, MICHAEL · 2016 to 2025
$18.9M
Training/Dissemination-Resource for Molecular Imaging Agents in Precision MedicineP41EB024495 · NIBIB · UT SOUTHWESTERN MEDICAL CENTER · PI MARTIN G POMPER · 2017 to 2026
$11.8M
Toxicology in the 21st Century Program (Tox21) - Systems ToxicologyZIATR000038 · NCATS · NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES · PI XIA, MENGHANG · 2015 to 2025
$5.7M
Measuring Intralesional Drug Exposures in Cavitary TB using Noninvasive In Vivo PET ImagingR01AI153349 · NIAID · JOHNS HOPKINS UNIVERSITY · PI JAIN, SANJAY · 2020 to 2024
$3.8M
ACE2-targeted PET radiotracers for investigating spatiotemporal distribution of SARS-CoV-2 organ injury and therapy response.R01AI161829 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI DESAI, TEJAL A., FLAVELL, ROBERT RICHARD · 2021 to 2023
$2.4M
NIAID NIH HHS R01 AI153349NIAID NIH HHS R01 AI161829NIBIB NIH HHS P41 EB024495
6 · The paper itself

Abstract

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein binds angiotensin-converting enzyme 2 (ACE2) at the cell surface, which constitutes the primary mechanism driving SARS-CoV-2 infection. Molecular interactions between the transduced S and endogenous proteins likely occur post-infection, but such interactions are not well understood. We used an unbiased primary screen to profile the binding of full-length S against >9,000 human proteins and found significant S-host protein interactions, including one between S and human estrogen receptor alpha (ERα). After confirming this interaction in a secondary assay, we used bioinformatics, supercomputing, and experimental assays to identify a highly conserved and functional nuclear receptor coregulator (NRC) LXD-like motif on the S2 subunit and an S-ERα binding mode. In cultured cells, S DNA transfection increased ERα cytoplasmic accumulation, and S treatment induced ER-dependent biological effects and ACE2 expression. Noninvasive multimodal PET/CT imaging in SARS-CoV-2-infected hamsters using [

Identifiers

PMID35665018
PMCPMC9164441
OpenAlexW4281388080

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.