Evidence map›Paper›PMID 35665006›Full record

ArticlebioRxiv : the preprint server for biology2022

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids.

Jessica M Vanslambrouck, Sean B Wilson, Ker Sin Tan, Ella Groenewegen, Rajeev Rudraraju, Jessica Neil, Kynan T Lawlor, Sophia Mah, Michelle Scurr, Sara E Howden and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Jessica M VanslambrouckMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Sean B WilsonMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Ker Sin TanMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Ella GroenewegenMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Rajeev RudrarajuDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, VIC, Australia.
Jessica NeilDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, VIC, Australia.
Kynan T LawlorMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Sophia MahMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Michelle ScurrMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Sara E HowdenMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
Kanta SubbaraoDepartment of Microbiology and Immunology, The Peter Doherty Institute for Infection and Immunity, The University of Melbourne, VIC, Australia.
Melissa H LittleMurdoch Children's Research Institute, Flemington Rd, Parkville, VIC, Australia.
The University of Melbourne · AUMurdoch Children's Research Institute · AU

Funding

Generating kidney organoids and mature renal cell types via directed iPSC differentiationUH3DK107344 · NIDDK · MURDOCH CHILDREN'S RESEARCH INSTITUTE · PI LITTLE, MELISSA HELEN · 2017 to 2019
$1.0M
NIDDK NIH HHS UH3 DK107344
6 · The paper itself

Abstract

While pluripotent stem cell-derived kidney organoids are now being used to model renal disease, the proximal nephron remains immature with limited evidence for key functional solute channels. This may reflect early mispatterning of the nephrogenic mesenchyme and/or insufficient maturation. Here we show that enhanced specification to metanephric nephron progenitors results in elongated and radially aligned proximalised nephrons with distinct S1 - S3 proximal tubule cell types. Such PT-enhanced organoids possess improved albumin and organic cation uptake, appropriate KIM-1 upregulation in response to cisplatin, and improved expression of SARS-CoV-2 entry factors resulting in increased viral replication. The striking proximo-distal orientation of nephrons resulted from localized WNT antagonism originating from the organoid stromal core. PT-enhanced organoids represent an improved model to study inherited and acquired proximal tubular disease as well as drug and viral responses.

Identifiers

PMID35665006
PMCPMC9164445
OpenAlexW3206771067

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.