Evidence map›Paper›PMID 35664651›Full record

Article3 Biotech2022

Development of amperometric biosensor based on cloned hemagglutinin gene of H1N1 (swine flu) virus.

Ravina, Paramjeet Singh Gill, Jagriti Narang, Ashok Kumar, Hari Mohan

Open access · bronzeAbstract read
In one paragraph

Article in 3 Biotech, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. TiOMikrochimica acta · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

RavinaCentre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana 124007 India.
Paramjeet Singh GillPt.Bhagwat Dyal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana 124001 India.
Jagriti NarangDepartment of Biotechnology, Jamia Hamdard, Delhi, India.
Ashok KumarCSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007 India.
Hari MohanCentre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, Haryana 124007 India.ORCID 0000-0003-1990-7238
Maharshi Dayanand University · INInstitute of Genomics and Integrative Biology · INJamia Hamdard · INPandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The recent emergence of respiratory viruses especially COVID-19 and swine flu has underscored the need for robust and bedside detection methods. Swine flu virus is a very infectious virus of the respiratory system. Timely detection of this virus with high specificity and sensitivity is crucial for reducing morbidity as well as mortality. Cloning of gene segments into a non-infectious agent helps in the development of detection methods, vaccine development, and other studies. In this study, cloning was used to develop a biosensor for H1N1 pdm09 detection. A segment of the hemaglutinin gene was cloned in a vector and characterized with the help of colony touch PCR and blue-white screening. The recombinant plasmid was extracted, and the gene segment was confirmed with the help of HA-specific primers. A 5' amine group-attached hemagglutinin (HA) gene-specific DNA probe was immobilized on the working gold electrode surface to make a quick, specific, reliable, and sensitive detection method for H1N1pdm09 virus in human nasal swab samples. The HA probe was immobilized on the cysteine applied gold electrode of the screen-printed electrode through 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and

Indexed as

BiosensorE. coli DH5αH1N1Hemagglutinin geneTA cloning

Identifiers

PMID35664651
PMCPMC9156826
OpenAlexW4281850906

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.