Evidence map›Paper›PMID 35662380›Full record

ArticleArchives of microbiology2022

Biofilm formation, antimicrobial assay, and toxin-genotypes of Clostridium perfringens type C isolates cultured from a neonatal Yangtze finless porpoise.

Jia Li, Richard William McLaughlin, Yingli Liu, Junying Zhou, Xueying Hu, Xiaoling Wan, Haixia Xie, Yujiang Hao, Jinsong Zheng

Abstract read
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In one paragraph

Article in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Jia Li *Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Richard William McLaughlin *General Studies, Gateway Technical College, Kenosha, WI, USA.
Yingli LiuInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Junying ZhouDepartment of Clinical Laboratory, Zhongnan Hospital of Wuhan University, Wuhan, 430072, China.
Xueying HuCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.
Xiaoling WanInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Haixia XieInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yujiang HaoInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Jinsong ZhengInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. zhengjinsong@ihb.ac.cn.ORCID http://orcid.org/0000-0002-6541-1594
Chinese Academy of Sciences · CNGateway Technical College · USHuazhong Agricultural University · CNInstitute of Hydrobiology · CNWuhan University · CN

Funding

National Natural Science Foundation of China 31870372Postdoctoral Research Foundation of China 2020M682530the Bureau of Science & Technology for Development, Chinese Academy of Sciences ZSSD-004
6 · The paper itself

Abstract

This is a culture-dependent study with the objective of pure culturing and characterizing pathogenic bacteria from the blowhole, lung, stomach and fecal samples of a neonatal crucially endangered Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis) that died 27 days after birth. Bacteria were inoculated using a swab onto blood and MacConkey agar plates and representative isolates were identified through 16S rRNA gene sequence analysis. A total of three Clostridium perfringens type C strains from the fecal samples were isolated. Toxin genes, including cpa, cpb and cpb2, were detected by PCR amplification, whereas the etx, iap and cpe genes were not detected. Biofilm formation of the three strains was then examined. Only one strain was capable of biofilm formation. In addition, isolates showed strong resistance against the antibiotics amikacin (3/3), erythromycin (1/3), gentamicin (3/3), streptomycin (3/3), and trimethoprim (3/3), while sensitivity to ampicillin (3/3), bacitracin (3/3), erythromycin (2/3), penicillin G (3/3), and tetracycline (3/3). The results suggested C. perfringens type C could have contributed to the death of this neonatal porpoise.

Indexed as

PorpoisesAnimalsAnti-Bacterial AgentsBacteriaBiofilmsClostridium perfringensErythromycinGenotypeRNA, Ribosomal, 16SAnti-Bacterial AgentsErythromycinRNA, Ribosomal, 16SAntibiotic resistanceBiofilm formationClostridium perfringens type CNeophocaena asiaeorientalis asiaeorientalisYangtze finless porpoise

Identifiers

PMID35662380
OpenAlexW4281730521

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.