ArticleWater research2022
Longitudinal SARS-CoV-2 RNA wastewater monitoring across a range of scales correlates with total and regional COVID-19 burden in a well-defined urban population.
Article in Water research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 2 of them syntheses that pooled it.
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Who cites it
36 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Influence of Sampling Strategies and Disease Prevalence on SARS-CoV-2 Detection Dynamics in Wastewater Surveillance.Viruses · 2026Article
- Article
- Designing effective SARS-CoV-2 wastewater surveillance system: lessons learnt from sustained monitoring in Delhi, India.BMC infectious diseases · 2026Article
- Assessing Urban Ditches for Tracking SARS-CoV-2 in Low Sanitation Areas in Southeastern Brazil.Food and environmental virology · 2026Article
- Implementation of SARS-CoV-2 Wastewater Surveillance Systems in Germany-Pilot Study in the Federal State of Thuringia.Microorganisms · 2026Article
- Article
- Article
- Performance and suitability of wastewater based-surveillance for SARS-CoV-2 RNA in public schools.Scientific reports · 2025Article
- Long term assessment of SARS-CoV-2 in wastewater and the transition to evaluate additional viral targets.The Science of the total environment · 2025Article
- Article
- Advancing health equity in wastewater-based epidemiology: A global critical review and conceptual framework.SSM - population health · 2025Review
- Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods.Communications medicine · 2025Article
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- Public health actions in response to pathogen detection in wastewater and the environment: a scoping review.Frontiers in public health · 2025Article
- Correlation between Viral Wastewater Concentration and Respiratory Tests, Oregon, USA.Emerging infectious diseases · 2024Article
- Article
- SARS-CoV-2 wastewater surveillance in the Czech Republic: Spatial and temporal differences in SARS-CoV-2 RNA concentrations and relationship to clinical data and wastewater parameters.Water research X · 2024Article
- A Bayesian framework for modeling COVID-19 case numbers through longitudinal monitoring of SARS-CoV-2 RNA in wastewater.Statistics in medicine · 2024Article
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33 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based epidemiology (WBE) is an emerging surveillance tool that has been used to monitor the ongoing COVID-19 pandemic by tracking SARS-CoV-2 RNA shed into wastewater. WBE was performed to monitor the occurrence and spread of SARS-CoV-2 from three wastewater treatment plants (WWTP) and six neighborhoods in the city of Calgary, Canada (population 1.44 million). A total of 222 WWTP and 192 neighborhood samples were collected from June 2020 to May 2021, encompassing the end of the first-wave (June 2020), the second-wave (November end to December 2020) and the third-wave of the COVID-19 pandemic (mid-April to May 2021). Flow-weighted 24-hour composite samples were processed to extract RNA that was then analyzed for two SARS-CoV-2-specific regions of the nucleocapsid gene, N1 and N2, using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Using this approach SARS-CoV-2 RNA was detected in 98.06% (406/414) of wastewater samples. SARS-CoV-2 RNA abundance was compared to clinically diagnosed COVID-19 cases organized by the three-digit postal code of affected individuals' primary residences, enabling correlation analysis at neighborhood, WWTP and city-wide scales. Strong correlations were observed between N1 & N2 gene signals in wastewater and new daily cases for WWTPs and neighborhoods. Similarly, when flow rates at Calgary's three WWTPs were used to normalize observed concentrations of SARS-CoV-2 RNA and combine them into a city-wide signal, this was strongly correlated with regionally diagnosed COVID-19 cases and clinical test percent positivity rate. Linked census data demonstrated disproportionate SARS-CoV-2 in wastewater from areas of the city with lower socioeconomic status and more racialized communities. WBE across a range of urban scales was demonstrated to be an effective mechanism of COVID-19 surveillance.
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