Evidence map›Paper›PMID 35660769›Full record

ArticleScientific reports2022

Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study.

Karolina Pierzchała, Marlena Pięta, Monika Rola, Małgorzata Świerczyńska, Angelika Artelska, Karolina Dębowska, Radosław Podsiadły, Jakub Pięta, Jacek Zielonka, Adam Sikora and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
  2. Article
  3. Myeloperoxidase as a therapeutic target for oxidative damage in Alzheimer's disease.Journal of enzyme inhibition and medicinal chemistry · 2025
    Review
  4. Article
  5. Article
  6. Small molecule probes for peroxynitrite detection.Redox biochemistry and chemistry · 2024
    Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Karolina PierzchałaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Marlena PiętaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Monika RolaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Małgorzata ŚwierczyńskaDepartment of Chemistry, Institute of Polymer and Dye Technology, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland.
Angelika ArtelskaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Karolina DębowskaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Radosław PodsiadłyDepartment of Chemistry, Institute of Polymer and Dye Technology, Lodz University of Technology, Stefanowskiego 12/16, 90-924, Lodz, Poland.
Jakub PiętaDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Jacek ZielonkaDepartment of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA. jzielonk@mcw.edu.ORCID 0000-0002-2524-0145
Adam SikoraDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Andrzej MarcinekDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland.
Radosław MichalskiDepartment of Chemistry, Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924, Lodz, Poland. radoslaw.michalski@p.lodz.pl.ORCID 0000-0001-5601-0839
Lodz University of Technology · PLMedical College of Wisconsin · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MPO-derived oxidants including HOCl contribute to tissue damage and the initiation and propagation of inflammatory diseases. The search for small molecule inhibitors of myeloperoxidase, as molecular tools and potential drugs, requires the application of high throughput screening assays based on monitoring the activity of myeloperoxidase. In this study, we have compared three classes of fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid, including boronate-, aminophenyl- and thiol-based fluorogenic probes and we show that all three classes of probes are suitable for this purpose. However, probes based on the coumarin fluorophore turned out to be not reliable indicators of the inhibitors' potency. We have also determined the rate constants of the reaction between HOCl and the probes and they are equal to 1.8 × 10

Indexed as

Hypochlorous AcidPeroxidaseBoronic AcidsCoumarinsFluoresceinsFluorescent DyesBoronic AcidsCoumarinsFluoresceinsFluorescent DyesHypochlorous AcidPeroxidase

Identifiers

PMID35660769
PMCPMC9166712
OpenAlexW4281967558

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.