Evidence map›Paper›PMID 35659286›Full record

ArticleBMC research notes2022

Horizontal transfer of a plasmid possessing mcr-1 marked with a single nucleotide mutation between Escherichia coli isolates from community residents.

Yoshimasa Yamamoto, Ayano Higashi, Kanoko Ikawa, Hoa Thi Thanh Hoang, Takahiro Yamaguchi, Ryuji Kawahara, Hideki Noguchi, Thang Nam Nguyen, Diep Thi Khong, Hoa Thi Tran

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. The battle against colistin-resistantNew microbes and new infections · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Yoshimasa YamamotoUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan. yyamamot@gifu-u.ac.jp.ORCID http://orcid.org/0000-0002-2942-1672
Ayano HigashiUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
Kanoko IkawaUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
Hoa Thi Thanh HoangUnited Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, Gifu, Japan.
Takahiro YamaguchiDepartment of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
Ryuji KawaharaDepartment of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
Hideki NoguchiJoint Support-Center for Data Science Research, Research Organization of Information and Systems, Mishima, Japan.
Thang Nam NguyenCenter for Medical and Pharmaceutical Research and Service, Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam.
Diep Thi KhongCenter for Medical and Pharmaceutical Research and Service, Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam.
Hoa Thi TranCenter for Medical and Pharmaceutical Research and Service, Thai Binh University of Medicine and Pharmacy, Thai Binh, Vietnam.
Gifu University · JPThai Binh University of Medicine and Pharmacy · VNOsaka Prefectural Institute of Public Health · JPResearch Organization of Information and Systems · JP

Funding

Research Organization of Information and Systems 012RP2021The Japan Society for the Promotion of Science 20H00561
6 · The paper itself

Abstract

objectivesThe widespread dissemination of phenotypic colistin-resistant (COR) bacteria in the community threatens public health. The horizontal gene transfer of the mobile colistin resistance gene via plasmids is thought to be one of the main mechanisms for dissemination. However, genotypic evidence to prove this in community settings is limited. This study used genome analysis to demonstrate the direct horizontal colistin resistance gene transfer via plasmids in isolates from the community.

resultsA total of 19 isolates of COR Escherichia coli from stool specimens of 23 residents from seven households in the Vietnamese community were assessed in this study. The whole-genome sequence data of isolates were acquired using a combination of DNBSEQ short-reads and Nanopore long-read sequencing. Analysis of genomic data was performed using online tools such as Geneious. Analysis of the genomic information of COR E. coli isolates revealed that the isolates from two residents of different households had a similar IncP1 plasmid possessing mcr-1.1, marked with a single nucleotide mutation at the same position. The study provided direct evidence to prove that mcr was horizontally transmitted among bacteria in community residents.

Indexed as

Escherichia coli InfectionsEscherichia coli ProteinsAnti-Bacterial AgentsColistinDrug Resistance, BacterialEscherichia coliHumansMicrobial Sensitivity TestsMutationNucleotidesPlasmidsAnti-Bacterial AgentsColistinEscherichia coli ProteinsMCR-1 protein, E coliNucleotidesColistin resistanceEscherichia coliHorizontal gene transfermcr

Identifiers

PMID35659286
PMCPMC9166650
OpenAlexW4281974186

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.