Evidence map›Paper›PMID 35657927›Full record

ArticlePloS one2022

Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.

Siobhan Goss, Carlos Barba Bazan, Kaitlyn Neuman, Christina Peng, Nola Begeja, Celeste Elisabeth Suart, Ray Truant

Abstract read
In one paragraph

Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Poly ADP-ribose signaling is dysregulated in Huntington disease.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Siobhan GossDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0002-8518-7534
Carlos Barba BazanDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
Kaitlyn NeumanDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0001-7699-9680
Christina PengDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0001-7471-9059
Nola BegejaDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
Celeste Elisabeth SuartDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0003-1949-9377
Ray TruantDepartment of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.ORCID 0000-0003-2542-6641

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability.

Indexed as

MicroscopyPrinting, Three-DimensionalPlasticsPrintingReproducibility of ResultsPlastics

Identifiers

PMID35657927
PMCPMC9165904

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.