Evidence map›Paper›PMID 35657526›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Characterization of RBPome in Oxidative Stress Conditions.

Zhicheng Zhang, Evy Timmerman, Francis Impens, Frank Van Breusegem

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhicheng ZhangDepartment of Plant Biotechnology and Bioinformatics, Ghent, Belgium.
Evy TimmermanVIB Center for Medical Biotechnology, Ghent, Belgium.
Francis ImpensVIB Center for Medical Biotechnology, Ghent, Belgium.
Frank Van BreusegemVIB-UGent Center for Plant Systems Biology, Ghent, Belgium. frbre@psb.ugent.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cellular redox signaling is triggered by accumulation of various reactive oxygen species (ROS) that integrate with other signaling cascades to enable plants to ultimately respond to (a)biotic stresses. The identification of key regulators underlying redox signaling networks is therefore of high priority. This chapter describes an improved mRNA interactome capture method that allows to systematically detect oxidative stress responsive regulators in the post-transcriptional gene regulation (PTGR) pathway. The protocol includes PSB-D suspension cell culture preparation, setup of oxidative stress conditions, short-term exposure to UV irradiation, cell lysis, pull-down and purification of crosslinked messenger ribonucleoproteins, their mass spectrometric analyses, and identification of proteome by statistical analyses. As result, a comprehensive inventory of the functional oxidative stress responsive RBPome (OxRBPome) is generated, which paves the way toward new insights into PTGR processes in redox signaling.

Indexed as

Oxidative StressProteomeOxidation-ReductionPlantsReactive Oxygen SpeciesProteomeReactive Oxygen SpeciesIn vivo UV crosslinkingMass spectrometryOxidative stressPost-transcriptional gene regulationProteomicsRNA-binding proteins

Identifiers

PMID35657526

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.