Evidence map›Paper›PMID 35657481›Full record

ArticleBiotechnology journal2022

Production of novel SARS-CoV-2 Spike truncations in Chinese hamster ovary cells leads to high expression and binding to antibodies.

Shiaki A Minami, Seongwon Jung, Yihan Huang, Bradley S Harris, Matthew W Kenaston, Roland Faller, Somen Nandi, Karen A McDonald, Priya S Shah

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shiaki A MinamiDepartment of Chemical Engineering, University of California, California, Davis, USA.
Seongwon JungDepartment of Chemical Engineering, University of California, California, Davis, USA.
Yihan HuangDepartment of Materials Science and Engineering, University of California, California, Davis, USA.
Bradley S HarrisDepartment of Chemical Engineering, University of California, California, Davis, USA.
Matthew W KenastonDepartment of Microbiology & Molecular Genetics, University of California, California, Davis, USA.
Roland FallerDepartment of Chemical Engineering, University of California, California, Davis, USA.
Somen NandiDepartment of Chemical Engineering, University of California, California, Davis, USA.
Karen A McDonaldDepartment of Chemical Engineering, University of California, California, Davis, USA.
Priya S ShahDepartment of Chemical Engineering, University of California, California, Davis, USA.ORCID https://orcid.org/0000-0001-7518-2839

Funding

QE-HF-X Mass SpectrometerS10OD026918 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2020 to 2020
$600k
NIH HHS S10 OD026918
6 · The paper itself

Abstract

SARS-CoV-2 Spike is a key protein that mediates viral entry into cells and elicits antibody responses. Its importance in infection, diagnostics, and vaccinations has created a large demand for purified Spike for clinical and research applications. Spike is difficult to express, prompting modifications to the protein and expression platforms to improve yields. Alternatively, the Spike receptor-binding domain (RBD) is commonly expressed with higher titers, though it has lower sensitivity in serological assays. Here, we improve transient Spike expression in Chinese hamster ovary (CHO) cells. We demonstrate that Spike titers increase significantly over the expression period, maximizing at 14 mg L

Indexed as

COVID-19SARS-CoV-2AnimalsAntibodies, NeutralizingAntibodies, ViralCHO CellsCricetinaeCricetulusSpike Glycoprotein, CoronavirusAntibodies, NeutralizingAntibodies, ViralSpike Glycoprotein, Coronavirusspike protein, SARS-CoV-2antibody bindingChinese hamster ovary cellsCOVID-19SARS-CoV-2Spike

Identifiers

PMID35657481
PMCPMC9347691

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.