Evidence map›Paper›PMID 35657453›Full record

ArticleMolecular biology reports2022

Characterization of 58 STRs and 94 SNPs with the ForenSeq™ DNA signature prep kit in Mexican-Mestizos from the Monterrey city (Northeast, Mexico).

José Alonso Aguilar-Velázquez, Miguel Ángel Duran-Salazar, Miranda Fabiola Córdoba-Mercado, Carolina Elena Coronado-Avila, Orlando Salas-Salas, Gabriela Martinez-Cortés, Ferrán Casals, Francesc Calafell, Benito Ramos-González, Héctor Rangel-Villalobos

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

José Alonso Aguilar-Velázquez *Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad #1115, CP 47810, Ocotlán, Jalisco, Mexico.ORCID http://orcid.org/0000-0002-5346-5595
Miguel Ángel Duran-Salazar *Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad #1115, CP 47810, Ocotlán, Jalisco, Mexico.
Miranda Fabiola Córdoba-MercadoInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey, Nuevo León, Mexico.
Carolina Elena Coronado-AvilaInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey, Nuevo León, Mexico.
Orlando Salas-SalasInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey, Nuevo León, Mexico.
Gabriela Martinez-CortésInstituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad #1115, CP 47810, Ocotlán, Jalisco, Mexico.ORCID http://orcid.org/0000-0002-3947-4612
Ferrán CasalsServei de Genómica, Universitat Pampeu Fabra, Dr. Aiguader, 88, 08003, Barcelona, Spain.ORCID http://orcid.org/0000-0002-8941-0369
Francesc CalafellInstitut de Biologia Evolutiva, Universitat Pompeu Fabra, Dr. Aiguader, 88, 08003, Barcelona, Spain.ORCID http://orcid.org/0000-0002-1083-9438
Benito Ramos-GonzálezInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey, Nuevo León, Mexico. benito.ramos@gmail.com.
Héctor Rangel-VillalobosInstituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad #1115, CP 47810, Ocotlán, Jalisco, Mexico. hrangel13@hotmail.com.ORCID http://orcid.org/0000-0003-2641-8782
Universidad de Guadalajara · MXUniversitat Pompeu Fabra · ESUniversidad Autónoma de Nuevo León · MX

Funding

Consejo Nacional de Ciencia y Tecnología 286623
6 · The paper itself

Abstract

backgroundSTR allele frequency databases from populations are necessary to take full advantage of the increased power of discrimination offered by massively parallel sequencing (MPS) platforms. MATERIAL AND

methodsFor this reason, we sequenced 58 STRs (aSTRs, X-STRs, and Y-STRs) and 94 identity informative SNPs (iiSNPs) on 105 Mestizo (admixed) individuals from Monterrey City (Northeast, Mexico), with the Primer Set-A of the ForenSeq™ DNA Signature Prep Kit.

resultsMost of the STR markers were in Hardy Weinberg equilibrium, with a few exceptions. We found 346 different length-based alleles for these 58 STRs; nevertheless, they became 528 alleles when the sequence was assessed. The combined power of discrimination from autosomal STRs (aSTRs) was -virtually- 100% in both length and sequence-based alleles, while the power of exclusion was 99.9999999976065 and 99.9999999999494%, respectively. Haplotypes based on X-STRs and Y-STRs showed 100% of discriminatory capacity.

conclusionsThese results provide -for the first time- forensic genomic population data from Mexico necessary for interpretation in kinship and criminal analyses.

Indexed as

DNA FingerprintingPolymorphism, Single NucleotideDNAGene FrequencyHigh-Throughput Nucleotide SequencingHumansMexicoMicrosatellite RepeatsSequence Analysis, DNADNAForenSeq DNA signature PrepMexican populationMiSeq FGxMPSVerogen

Identifiers

PMID35657453
OpenAlexW4281817692

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.