ArticleAngewandte Chemie (International ed. in English)2022
Discovery and Genetic Code Expansion of a Polyethylene Terephthalate (PET) Hydrolase from the Human Saliva Metagenome for the Degradation and Bio-Functionalization of PET.
Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- The diversity of PET degrading enzymes: A systematic review of sequence, structure, and function.Protein science : a publication of the Protein Society · 2025Pooled it
- Enhancing the Versatility of Polyethylene Terephthalate (PET) Through Strategic Biomolecular Functionalization.Angewandte Chemie (International ed. in English) · 2026Review
- Deep Learning-Driven Discovery and Engineering of an Efficient PETase for Depolymerization and Detoxification of PET Microplastics Under Physiological Conditions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- EngineeringInternational journal of molecular sciences · 2025Review
- Harnessing protein language model for structure-based discovery of highly efficient and robust PET hydrolases.Nature communications · 2025Article
- Click biology highlights the opportunities from reliable biological reactions.Nature chemical biology · 2025Review
- Standardization guidelines and future trends for PET hydrolase research.Nature communications · 2025Review
- Incorporation of polylactic acid microplastics into the carbon cycle as a carbon source to remodel the endogenous metabolism of the gut.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Replicating PET Hydrolytic Activity by Positioning Active Sites with Smaller Synthetic Protein Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Genetic Code Expansion: Recent Developments and Emerging Applications.Chemical reviews · 2025Review
- Plastic degradation by enzymes from uncultured deep sea microorganisms.The ISME journal · 2025Article
- Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integratedSynthetic and systems biotechnology · 2025Article
- Perspectives on the microorganisms with the potentials of PET-degradation.Frontiers in microbiology · 2025Review
- Tuning the performance of a TphR-based terephthalate biosensor with a design of experiments approach.Metabolic engineering communications · 2024Article
- Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming.Chemical reviews · 2024Review
- Engineered polyethylene terephthalate hydrolases: perspectives and limits.Applied microbiology and biotechnology · 2024Review
- Nature's Plastic Predators: A Comprehensive and Bibliometric Review of Plastivore Insects.Polymers · 2024Review
- The Impact of Metagenomics on Biocatalysis.Angewandte Chemie (International ed. in English) · 2024Review
- Molecular Insights into the Enhanced Activity and/or Thermostability of PET Hydrolase by D186 Mutations.Molecules (Basel, Switzerland) · 2024Article
- Polyethylene Terephthalate Hydrolases in Human Gut Microbiota and Their Implications for Human Health.Microorganisms · 2024Article
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 3 countries.
Funding
Abstract
We report a bioinformatic workflow and subsequent discovery of a new polyethylene terephthalate (PET) hydrolase, which we named MG8, from the human saliva metagenome. MG8 has robust PET plastic degradation activities under different temperature and salinity conditions, outperforming several naturally occurring and engineered hydrolases in degrading PET. Moreover, we genetically encoded 2,3-diaminopropionic acid (DAP) in place of the catalytic serine residue of MG8, thereby converting a PET hydrolase into a covalent binder for bio-functionalization of PET. We show that MG8(DAP), in conjunction with a split green fluorescent protein system, can be used to attach protein cargos to PET as well as other polyester plastics. The discovery of a highly active PET hydrolase from the human metagenome-currently an underexplored resource for industrial enzyme discovery-as well as the repurposing of such an enzyme into a plastic functionalization tool, should facilitate ongoing efforts to degrade and maximize reusability of PET.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.