Evidence map›Paper›PMID 35656648›Full record

ArticleElectrophoresis2022

Rapid, inexpensive fabrication of electrophoretic microdevices for fluorescence detection.

Daniel A Nelson, Brandon L Thompson, An-Chi Scott, Renna Nouwairi, Christopher Birch, Jacquelyn A DuVall, Delphine Le Roux, Jingyi Li, Brian E Root, James P Landers

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrophoresis, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Daniel A NelsonDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Brandon L ThompsonDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
An-Chi ScottDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Renna NouwairiDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.ORCID 0000-0003-4013-3126
Christopher BirchDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Jacquelyn A DuVallDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Delphine Le RouxDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Jingyi LiDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
Brian E RootDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
James P LandersDepartment of Chemistry, University of Virginia, Charlottesville, Virginia, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The laser print, cut, and laminate (PCL) method for microfluidic device fabrication can be leveraged for rapid and inexpensive prototyping of electrophoretic microchips useful for optimizing separation conditions. The rapid prototyping capability allows the evaluation of fluidic architecture, applied fields, reagent concentrations, and sieving matrix, all within the context of using fluorescence-compatible substrates. Cyclic olefin copolymer and toner-coated polyethylene terephthalate (tPeT) were utilized with the PCL technique and bonding methods optimized to improve device durability during electrophoresis. A series of separation channel designs and centrifugation conditions that provided successful loading of sieving polymer in less than 3 min was described. Separation of a 400-base DNA sizing ladder provided calculated base resolution between 3 and 4 bases, a greater than 18-fold improvement over separations on similar substrates. Finally, the accuracy and precision capabilities of these devices were demonstrated by separating and sizing DNA fragments of 147 and 167 bases as 148.62 ± 2 and 166.48 ± 3 bases, respectively.

Indexed as

DNALab-On-A-Chip DevicesCentrifugationElectrophoresisPolymersDNAPolymersdeoxyribonucleic acidDNAelectrophoresis

Identifiers

PMID35656648
PMCPMC9544361

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.