Evidence map›Paper›PMID 35655187›Full record

ArticleMicrobial cell factories2022

Overlapping promoter library designed for rational heterogenous expression in Cordyceps militaris.

Mengdi Lyu, Jiapeng Zeng, Yue Zhou, Tongyu Zhang, Aiping Wang, Jiezhao Ma, Ziyi Wu, Alvaro Castells-Garcia, Esther González-Almela, Junfang Lin and 1 more

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Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mengdi LyuDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Jiapeng ZengDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Yue ZhouDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Tongyu ZhangDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Aiping WangBioland Laboratory, Guangzhou, 510005, China.
Jiezhao MaDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Ziyi WuDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Alvaro Castells-GarciaBioland Laboratory, Guangzhou, 510005, China.
Esther González-AlmelaBioland Laboratory, Guangzhou, 510005, China.
Junfang LinDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China.
Tao WeiDepartment of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou, 510640, Guangdong, China. weitao@scau.edu.cn.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province No. 2019A1515110609Scientific Research Platform Project of Education Department of Guangdong Province No. 2020KTSCX019
6 · The paper itself

Abstract

backgroundCordyceps militaris, a kind of edible and medicinal fungus widely accepted in East Asia, has attracted much attention as a potential cell factory for producing adenosine analogs. Despite the rapid development in gene editing techniques and genome modeling, the diversity of DNA elements in C. militaris was too short to achieve rational heterogeneous expression for metabolic engineering studies.

resultsIn this study, P

conclusionsThis study firstly expanded the overlapping promoter strategy used in model microorganism in C. militaris. It was a proof-of-concept in fungi synthetic biology and provide a general method to pushed the boundary of promoter engineering in edible mushroom.

Indexed as

CordycepsCloning, MolecularGene LibraryGenes, ReporterPromoter Regions, GeneticBioBricksCordyceps militarisFungiOverlapping promoter

Identifiers

PMID35655187
PMCPMC9161592

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.