Evidence map›Paper›PMID 35655031›Full record

ArticleTropical animal health and production2022

Molecular detection of porcine parvovirus 1-associated reproductive failure in southern India.

S Parthiban, R K V Sowndhraya, P Raja, M Parthiban, A Ramesh, G Dhinakar Raj, K Senthilkumar, D Balasubramanyam, S Hemalatha, R Bharathi and 2 more

Abstract read
In one paragraph

Article in Tropical animal health and production, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

S ParthibanDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India. parthis17@gmail.com.
R K V SowndhrayaSree Sastha Institute of Engineering and Technology, Chennai, India.
P RajaDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India.
M ParthibanDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India.
A RameshVaccine Research Centre-Viral Vaccines, CAHS, MMC, Chennai-51, India.
G Dhinakar RajDepartment of Animal Biotechnology, Faculty of Basic Sciences, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600 007, India.
K SenthilkumarPost Graduate Research Institute in Animal Sciences, Kattupakkam, India.
D BalasubramanyamPost Graduate Research Institute in Animal Sciences, Kattupakkam, India.
S HemalathaDepartment of Veterinary Pathology, MVC, Chennai, India.
R BharathiCentral University Laboratory, CAHS, MMC, Chennai, India.
Chintu RavishankarDepartment of Veterinary Microbiology, COVAS, Pookode, Kerala Veterinary and Animal Sciences University, Kerala, India.
S Thahira ParveenJeppiaar Engineering College, Chennai, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study used 56 aborted and stillborn fetuses from organized swine farms in Tamil Nadu and Kerala, southern states of India. All samples were screened by using a PCR assay that targets the NS1 gene for PPV. Furthermore, the PCR positive samples were subjected to amplification of the VP2 gene of PPV1 with designed primers and sequenced for further study. The PCR screening of 56 samples found that 14.3% (n = 8) were positive for PPV genome. According to VP2 gene-based PCR for PPV1, 897 bp specific amplicons were detected in all eight of the samples. Two of the eight positive samples (L17 and T5) were sequenced and annotated randomly. The BLAST analysis of contig sequence INDTNCHN-T5 revealed 100% sequence homology with Chinese PPV1genome, whereas sequence from INDTNCHN-L17 revealed 99.43% sequence homology with Spain, Chinese, and German. PPV1 sequences and both the sequences INDTNCHN-T5 and INDTNCHN-L17 were submitted to the GenBank under the accession numbers MW822566 and MW822567 respectively. A phylogenetic analysis of the sequences in this study revealed specific grouping along with PPV1 strains in cluster E. Amino acid analysis of both isolated sequences in addition to the reference sequence from PPV1 showed variations in position 215 (I to T) in both the isolates, variation at position 228 (Q to E) in T5 isolate and variations at position 59 (L to M) and 314 (K to E) in L17 isolate. This study represents the first report of PPV1 cluster E in Tamil Nadu, southern India.

Indexed as

Parvovirus, PorcineAnimalsDNA, ViralIndiaPhylogenyPolymerase Chain ReactionSwineDNA, ViralCharacterizationMolecular detectionPorcine parvovirus 1Reproductive failure

Identifiers

PMID35655031
PMCPMC9162486

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.