Evidence map›Paper›PMID 35650304›Full record

ArticlePsychopharmacology2022

Augmented anandamide signalling in the substantia nigra pars reticulata mediates panicolytic-like effects in mice confronted by Crotalus durissus terrificus pit vipers.

Rafael C Almada, Luiz Luciano Falconi-Sobrinho, Juliana A da Silva, Carsten T Wotjak, Norberto C Coimbra

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In one paragraph

Article in Psychopharmacology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 12 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Rafael C AlmadaLaboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Luiz Luciano Falconi-SobrinhoLaboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Juliana A da SilvaLaboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil.
Carsten T WotjakLaboratory of Neuronal Plasticity, Department of Stress Neurobiology and Neurogenetics, Max Planck Institute of Psychiatry, Munich, Germany.
Norberto C CoimbraLaboratory of Neuroanatomy and Neuropsychobiology, Department of Pharmacology, Ribeirão Preto Medical School of the University of São Paulo (FMRP-USP), Av. Bandeirantes, 3900, Ribeirão Preto, São Paulo, 14049-900, Brazil. nccoimbr@fmrp.usp.br.ORCID http://orcid.org/0000-0002-4676-2620
Universidade de Ribeirão Preto · BRUniversidade de São Paulo · BRBoehringer Ingelheim (Germany) · DE

Funding

Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico 470119/2004-7Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico 474853/2013-6Conselho Nacional de Pesquisa e Desenvolvimento Tecnológico 483763/2010-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2007/01174-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2012/03798-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/11855-8Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/03898-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/15050-7German-Israeli Foundation for Scientific Research and Development I-1442-421.13/2017Pro-Rectory of the University of São Paulo (USP) Research Grant IaPq2012-156-USP-12.1.25440.01.6
6 · The paper itself

Abstract

rationaleThe endocannabinoid modulation of fear and anxiety due to the on-demand synthesis and degradation is supported by a large body of research. Although it has been proposed that anandamide (AEA) in the substantia nigra pars reticulata (SNpr) seems to be important for the organisation of innate fear-related behaviours, a role for endogenous AEA has yet to be clarified.

methodsMice were treated with the fatty acid amide hydrolase (FAAH) selective inhibitor URB597 at different concentrations (0.01, 0.1, 1 nmol/0.1 µL) in the SNpr and confronted by rattlesnakes (Crotalus durissus terrificus). The most effective dose of URB597 (1 nmol) was also preceded by microinjections of the CB

resultsURB597 (0.1 and 1 nmol) in the SNpr decreased the expression of defensive behaviours such as defensive attention, escape, and time spent inside the burrow of mice confronted by rattlesnakes. Moreover, pretreatment of SNpr with AM251 suppressed these antiaversive effects of URB597 in this midbrain structure.

conclusionOverall, these data clearly indicate that the panicolytic consequences of endogenous AEA enhancement in the SNpr are mediated by CB1 receptor signalling.

Indexed as

CrotalinaePars ReticulataAnimalsArachidonic AcidsCrotalusEndocannabinoidsMicePolyunsaturated AlkamidesReceptor, Cannabinoid, CB1Substantia NigraanandamideArachidonic AcidsEndocannabinoidsPolyunsaturated AlkamidesReceptor, Cannabinoid, CB1AnandamideCrotalus durissus terrificus venomous pit viperPanic attacksPrey vs. venomous snake paradigmSubstantia nigra pars reticulata (SNpr)URB597

Identifiers

PMID35650304
OpenAlexW4281977326

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.