ArticleProceedings of the National Academy of Sciences of the United States of America2022
Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 71 citations in OpenAlex.
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- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.eLife · 2026Article
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- Revisiting Incomplete Tissue-Level Reperfusion Following Successful Thrombectomy for Acute Ischemic Stroke.Annals of neurology · 2026Review
- Microvascular architecture and physiological fluctuations constrain the control of cerebral microcirculation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Tunable Bessel beam two-photon fluorescence microscopy for high-speed volumetric imaging of brain dynamics.bioRxiv : the preprint server for biology · 2026Article
- Cortical Microvascular Pulsatility in the Aging Mouse Brain and the Confounding Effects of Anesthesia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-pixel imaging flow cytometry for biomedical research.Inflammation and regeneration · 2025Review
- Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025Article
- Vasomotion delineates cerebral vascular dynamic features and participates in the homeostatic cerebral blood flow regulation.Scientific reports · 2025Article
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Kilohertz volumetric imaging of in vivo dynamics using squeezed light field microscopy.Nature methods · 2025Article
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- Unlocking Multimodal Nonlinear Microscopy for Deep-Tissue Imaging under Continuous-Wave Excitation with Tunable Upconverting Nanoparticles.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Arteries and veins in awake mice using two-photon microscopy.Journal of anatomy · 2025Article
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7 authors at 4 institutions in 3 countries.
Funding
Abstract
Characterizing blood flow dynamics in vivo is critical to understanding the function of the vascular network under physiological and pathological conditions. Existing methods for hemodynamic imaging have insufficient spatial and temporal resolution to monitor blood flow at the cellular level in large blood vessels. By using an ultrafast line-scanning module based on free-space angular chirped enhanced delay, we achieved two-photon fluorescence imaging of cortical blood flow at 1,000 two-dimensional (2D) frames and 1,000,000 one-dimensional line scans per second in the awake mouse. This orders-of-magnitude increase in temporal resolution allowed us to measure cerebral blood flow at up to 49 mm/s and observe pulsatile blood flow at harmonics of heart rate. Directly visualizing red blood cell (RBC) flow through vessels down to >800 µm in depth, we characterized cortical layer–dependent flow velocity distributions of capillaries, obtained radial velocity profiles and kilohertz 2D velocity mapping of multifile blood flow, and performed RBC flux measurements from penetrating blood vessels.
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