ArticlePloS one2022
A Borrelia burgdorferi outer surface protein C (OspC) genotyping method using Luminex technology.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 9 citations in OpenAlex.
- Evaluation of immunoreactive epitopes in the sera and cerebrospinal fluid of patients with post-treatment Lyme disease syndrome.Scientific reports · 2026Article
- Molecular Detection and Characterization of Tick-Borne Pathogens inPathogens (Basel, Switzerland) · 2025Article
- Novel recombinant vaccinia virus-vectored vaccine affords complete protection against homologousEmerging microbes & infections · 2024Article
- Whole-genome sequencing of Western CanadianMicrobial genomics · 2024Article
- Population dynamics of the Lyme disease bacterium, Borrelia burgdorferi, during rapid range expansion in New York State.Molecular ecology · 2024Article
- Differential Resistance ofPathogens (Basel, Switzerland) · 2023Article
- Lyme borreliosis diagnosis: state of the art of improvements and innovations.BMC microbiology · 2023Review
- White-Tailed Deer Serum Kills the Lyme Disease Spirochete,Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2023Article
- Prevalence of Borrelia burgdorferi and diversity of its outer surface protein C (ospC) alleles in blacklegged ticks (Ixodes scapularis) in Delaware.Ticks and tick-borne diseases · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Borrelia burgdorferi is an important tickborne human pathogen comprising several strains based on nucleotide sequence of the outer surface protein C (ospC) gene. Detection and characterization of different ospC genotypes is vital for research on B. burgdorferi and the risk it poses to humans. Here we present a novel, multiplex assay based on Luminex xMAP technology for the detection of B. burgdorferi ospC genotypes. The assay has five major steps: amplification of the ospC gene, hydrolyzation of surplus primers and nucleotides, incorporation of biotinylated nucleotides into the template DNA, hybridization to Luminex microspheres, and detection of fluorescent signals corresponding to each ospC genotype. We validated the protocol by comparing results obtained from our method against results from an established ospC genotyping method. This protocol can be used for the characterization of ospC genotypes in B. burgdorferi infected ticks, reservoir hosts, and/or clinical samples.
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