Evidence map›Paper›PMID 35647917›Full record

ArticleActa crystallographica. Section D, Structural biology2022

Lipidic cubic phase serial femtosecond crystallography structure of a photosynthetic reaction centre.

Petra Båth, Analia Banacore, Per Börjesson, Robert Bosman, Cecilia Wickstrand, Cecilia Safari, Robert Dods, Swagatha Ghosh, Peter Dahl, Giorgia Ortolani and 25 more

Abstract read
In one paragraph

Article in Acta crystallographica. Section D, Structural biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Introduction to the virtual thematic issue on room-temperature biological crystallography.Acta crystallographica. Section F, Structural biology communications · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors.

Petra BåthDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.ORCID 0000-0002-8056-9250
Analia BanacoreDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Per BörjessonDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Robert BosmanDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Cecilia WickstrandDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Cecilia SafariDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Robert DodsDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Swagatha GhoshDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.ORCID 0000-0003-1063-0674
Peter DahlDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Giorgia OrtolaniDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Tinna Björg UlfarsdottirDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Greger HammarinDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
María José García BoneteDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.ORCID 0000-0002-8832-4338
Adams VallejosDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Lucija OstojićDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Petra EdlundDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Johanna Barbara LinseDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Rebecka AnderssonDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.
Eriko NangoRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Shigeki OwadaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.ORCID 0000-0002-1451-7612
Rie TanakaRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Kensuke TonoJapan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.ORCID 0000-0003-1218-3759
Yasumasa JotiJapan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5198, Japan.ORCID 0000-0002-4040-5972
Osamu NurekiDepartment of Biological Sciences, Graduate School of Science, University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
Fangjia LuoDepartment of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Daniel JamesSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.ORCID 0000-0002-8348-6661
Karol NassSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.ORCID 0000-0001-8843-1566
Philip J M JohnsonSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.
Gregor KnoppSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.ORCID 0000-0002-0786-3402
Dmitry OzerovSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.
Claudio CirelliSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.ORCID 0000-0003-4576-3805
Christopher MilneSwissFEL, Paul Scherrer Institute, Forschungsstrasse 111, CH-5232 Villigen PSI, Switzerland.ORCID 0000-0003-4714-9139
So IwataRIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Sayo-gun, Hyogo 679-5148, Japan.
Gisela BrändénDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.ORCID 0000-0001-5615-7187
Richard NeutzeDepartment of Chemistry and Molecular Biology, University of Gothenburg, Lundbergslaboratoriet Box 462, 405 30 Göteborg, Sweden.ORCID 0000-0003-0986-6153

Funding

European Commission NanoMemEuropean Commission X-probeHorizon 2020 Framework Programme 789030Japan Agency for Medical Research and Development JP21am0101070Japan Society for the Promotion of Science 19H05776Japan Society for the Promotion of Science 19H05781Vetenskapsrådet 2015-00560
6 · The paper itself

Abstract

Serial crystallography is a rapidly growing method that can yield structural insights from microcrystals that were previously considered to be too small to be useful in conventional X-ray crystallography. Here, conditions for growing microcrystals of the photosynthetic reaction centre of Blastochloris viridis within a lipidic cubic phase (LCP) crystallization matrix that employ a seeding protocol utilizing detergent-grown crystals with a different crystal packing are described. LCP microcrystals diffracted to 2.25 Å resolution when exposed to XFEL radiation, which is an improvement of 0.15 Å over previous microcrystal forms. Ubiquinone was incorporated into the LCP crystallization media and the resulting electron density within the mobile Q

Indexed as

Photosynthetic Reaction Center Complex ProteinsCrystallizationCrystallography, X-RayLipidsMembrane ProteinsUbiquinoneLipidsMembrane ProteinsPhotosynthetic Reaction Center Complex ProteinsUbiquinoneBlastochloris viridislipidic cubic phasemembrane-protein crystallizationmicrocrystalsphotosynthetic reaction centreserial crystallography

Identifiers

PMID35647917
PMCPMC9159286

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.