Evidence map›Paper›PMID 35647876›Full record

ArticleMicrobiology spectrum2022

Simple and Economical Extraction of Viral RNA and Storage at Ambient Temperature.

Sarah Hernandez, Fátima Cardozo, David R Myers, Alejandra Rojas, Jesse J Waggoner

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 16 citations in OpenAlex.

  1. Nipah virus molecular detection from whole blood and respiratory swabs in a rapid field-ready protocol.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2026
    Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Comprehensive Review on Viral RNA Extraction Strategies for Enhanced Molecular Diagnostics.Interdisciplinary perspectives on infectious diseases · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Sarah HernandezEmory University, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0002-0889-0998
Fátima CardozoUniversidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Departamento de Salud Pública, San Lorenzo, Paraguay.
David R MyersWallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, Atlanta, Georgia, USA.
Alejandra RojasUniversidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Departamento de Producción, San Lorenzo, Paraguay.
Jesse J WaggonerEmory University, Department of Medicine, Division of Infectious Diseases, Atlanta, Georgia, USA.ORCID 0000-0002-0244-5957
Emory University · USUniversidad Nacional de Asunción · PYGeorgia Institute of Technology · US

Funding

American Asian Centers for Arbovirus Research and Enhanced Surveillance (A2CARES)U01AI151788 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI Josefina Coloma, Eva Harris · 2020 to 2026
$9.6M
Detection and Characterization of Flavivirus and Alphavirus Infections in Acute Suspected Arboviral Cases or Neurological Disease, Central Department, ParaguayR21AI146443 · NIAID · EMORY UNIVERSITY · PI WAGGONER, JESSE · 2019 to 2020
$411k
NIAID NIH HHS R21 AI146443NIAID NIH HHS U01 AI151788
6 · The paper itself

Abstract

RNA extraction is essential for the molecular detection of common viral pathogens. However, available extraction methods and the need for ultra-cold storage limit molecular testing in resource-constrained settings. Herein, we describe the development of an economical

Indexed as

DengueRNA, ViralHumansMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionTemperatureRNA, Viraldengue virusextractionmolecular testingribonucleic acid

Identifiers

PMID35647876
PMCPMC9241768
OpenAlexW4281785792

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.