ArticleZhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics2022
[Features of intestinal flora in children with food protein-induced proctocolitis based on high-throughput sequencing].
Article in Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectivesTo study the features of intestinal flora in children with food protein-induced proctocolitis (FPIP) by high-throughput sequencing.
methodsA total of 31 children, aged <6 months, who experienced FPIP after exclusive breastfeeding and attended the outpatient service of the Third Affiliated Hospital of Zunyi Medical University from October 2018 to February 2021 were enrolled as the FPIP group. Thirty-one healthy infants were enrolled as the control group. Fecal samples were collected to extract DNA for PCR amplification. High-throughput sequencing was used to perform a bioinformatics analysis of 16S rDNA V3-V4 fragments in fecal samples.
resultsThe diversity analysis of intestinal flora showed that compared with the control group, the FPIP group had a lower Shannon index for diversity (
conclusionsCompared with the control group, the FPIP group has a reduction in the diversity of intestinal flora and an increase in their abundance, and there are certain differences in several bacterial genera. These results suggest that changes in the composition of intestinal flora at genus level may play an important role in the development and progression of FPIP.
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