Evidence map›Paper›PMID 35642012›Full record

ArticleBiomarker research2022

Extracellular miR-6723-5p could serve as a biomarker of limbal epithelial stem/progenitor cell population.

M Ruiz, S González, C Bonnet, S X Deng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomarker research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Multiple Stem/Progenitor Cells Isolated from the Limbus.Current stem cell research & therapy · 2025
    Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

M RuizCornea Division, Stein Eye Institute, University of California, 100 Stein Plaza, Los Angeles, CA, 90095, USA.
S GonzálezCornea Division, Stein Eye Institute, University of California, 100 Stein Plaza, Los Angeles, CA, 90095, USA.
C BonnetCornea Division, Stein Eye Institute, University of California, 100 Stein Plaza, Los Angeles, CA, 90095, USA.
S X DengCornea Division, Stein Eye Institute, University of California, 100 Stein Plaza, Los Angeles, CA, 90095, USA. deng@jsei.ucla.edu.

Funding

Vision Research Core at UCLAP30EY000331 · NEI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Xian-Jie Yang · 1985 to 2026
$16.8M
NEI NIH HHS P30 EY000331
6 · The paper itself

Abstract

backgroundDysfunction or loss of limbal stem cells can result in limbal stem cell deficiency (LSCD), a disease that cause corneal opacity, pain, and loss of vision. Cultivated limbal epithelial transplantation (CLET) can be used to restore stem cell niche homeostasis and replenish the progenitor pool. Transplantation has been reported with high success rate, but there is an unmet need of prognostic markers that correlate with clinical outcomes. To date, the progenitor content in the graft is the only parameter that has been retrospectively linked to success.

methodsIn this study, we investigate extracellular micro RNAs (miRNAs) associated with stem/progenitor cells in cultivated limbal epithelial cells (cLECs). Using micro RNA sequencing and linear regression modelling, we identify a miRNA signature in cultures containing high proportion of stem/progenitor cells. We then develop a robust RNA extraction workflow from culture media to confirm a positive miRNA correlation with stem/progenitor cell proportion.

resultsmiR-6723-5p is associated with cultures containing high proportion of stem/progenitor cells, and is detected in the basal layer of corneal epithelium.

conclusionsThese results indicate that miR-6723-5p could potentially serve as a stem/progenitor cell marker in cLECs.

Indexed as

BiomarkerCell therapyCorneaExplants cultureLimbal epitheliumLimbal stem cell deficiencyLimbal stem cellsmiR-6723-5pmiRNAsPotency assay

Identifiers

PMID35642012
PMCPMC9153202

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.