ArticleBiotechnology for biofuels and bioproducts2022
Genetic modifications of critical regulators provide new insights into regulation modes of raw-starch-digesting enzyme expression in Penicillium.
Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 22 citations in OpenAlex.
- A GTPase activating protein Polrg1 regulates the development and cellulase expression of Penicillium oxalicum.World journal of microbiology & biotechnology · 2025Article
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Mutual regulation of novel transcription factors RsrD and RsrE positively modulates the production of raw-starch-degrading enzyme inApplied and environmental microbiology · 2024Article
- A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.Communications biology · 2024Article
- Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A.Applied microbiology and biotechnology · 2024Article
- Glucose Catabolite Repression Participates in the Regulation of Sialidase Biosynthesis by Antarctic StrainJournal of fungi (Basel, Switzerland) · 2024Article
- Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex.Communications biology · 2023Article
- Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development.Applied microbiology and biotechnology · 2023Article
- Strain improvement ofFrontiers in microbiology · 2023Review
- Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum.Microbial cell factories · 2022Article
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9 authors at 2 institutions in 2 countries.
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Abstract
backgroundStarch is a very abundant and renewable carbohydrate and an important feedstock for industrial applications. However, most starch-based products are not cost-efficient due to the high energy input needed in traditional enzymatic starch conversion processes. Raw-starch-digesting enzymes (RSDEs) from filamentous fungi have great commercial value in starch processing. However, the regulatory mechanisms associated with their production in filamentous fungi remain unknown.
resultsIn this study, we reported the novel finding that cellulolytic fungus Penicillium oxalicum 114-2 has broad RSDE activity. Four regulators, including the amylase transcription activator AmyR, the catabolite repression repressor CreA, the group III G protein α subunit PGA3, and the nonhistone chromosomal protein HepA, have been found to play a crucial regulatory role in RSDE expression. Enzymatic assays revealed that RSDE production significantly increased after the overexpression of AmyR and HepA, the deletion of CreA and the dominant activation of PGA3. RT-qPCR analysis demonstrated that there is a mutual regulation mode between the four regulators, and then formed a cascade regulation mechanism that is involved in RSDE expression. Comparative transcriptomic analysis between the wild-type strain and genetically engineered strains revealed differentially expressed genes that may mediate the RSDE expression.
conclusionsThe four different types of regulators were systematically investigated and found to form a regulatory network controlling RSDE gene expression. Our results provide a new insight into the regulatory mechanism of fungal amylolytic enzyme expression and offer a theoretical basis to rationally improve the RSDE yield in the future.
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