ArticleACS nano2022
Controlling Nuclease Degradation of Wireframe DNA Origami with Minor Groove Binders.
Article in ACS nano, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- DNA Nanostructures for siRNA Delivery.Bioconjugate chemistry · 2026Review
- Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type Targeting.ACS applied materials & interfaces · 2026Article
- DNA origami vaccines program antigen-focused germinal centers.Science (New York, N.Y.) · 2026Article
- Advances and challenges in non-canonical nucleic acids data storage.Nature communications · 2026Review
- Resolving DNA origami structural integrity and pharmacokinetics in vivo.Nature nanotechnology · 2026Article
- Predicting the effect of binding molecules on the shape and mechanical properties of structured DNA assemblies.Nature communications · 2024Article
- Advancements in Aptamer-Driven DNA Nanostructures for Precision Drug Delivery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Improving DNA nanostructure stability: A review of the biomedical applications and approaches.International journal of biological macromolecules · 2024Review
- Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds.Nature communications · 2024Article
- Nano-Bio Interactions between DNA Nanocages and Human Serum Albumin.Journal of chemical theory and computation · 2023Article
- Peptide nanostructures-based delivery of DNA nanomaterial therapeutics for regulating gene expression.Molecular therapy. Nucleic acids · 2023Article
- Fluorometric Determination of DNA Nanostructure Biostability.ACS applied bio materials · 2023Article
- Enabling programmable dynamic DNA chemistry using small-molecule DNA binders.Nature communications · 2023Article
- Enhancing antibody responses by multivalent antigen display on thymus-independent DNA origami scaffolds.bioRxiv : the preprint server for biology · 2023Article
- Evaluation of Nonmodified Wireframe DNA Origami for Acute Toxicity and Biodistribution in Mice.ACS applied bio materials · 2023Article
- Evaluation of non-modified wireframe DNA origami for acute toxicity and biodistribution in mice.bioRxiv : the preprint server for biology · 2023Article
- Advancing the Utility of DNA Origami Technique through Enhanced Stability of DNA-Origami-Based Assemblies.Bioconjugate chemistry · 2023Review
- Innate Immune Stimulation Using 3D Wireframe DNA Origami.ACS nano · 2022Article
- Metallointercalators-DNA Tetrahedron Supramolecular Self-Assemblies with Increased Serum Stability.ACS nano · 2022Article
- Controlled Release in Hydrogels Using DNA Nanotechnology.Biomedicines · 2022Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
Viruslike particles (VLPs) fabricated using wireframe DNA origami are emerging as promising vaccine and gene therapeutic delivery platforms due to their programmable nature that offers independent control over their size and shape, as well as their site-specific functionalization. As materials that biodegrade in the presence of endonucleases, specifically DNase I and II, their utility for the targeting of cells, tissues, and organs depends on their stability in vivo. Here, we explore minor groove binders (MGBs) as specific endonuclease inhibitors to control the degradation half-life of wireframe DNA origami. Bare, unprotected DNA-VLPs composed of two-helix edges were found to be stable in fetal bovine serum under typical cell culture conditions and in human serum for 24 h but degraded within 3 h in mouse serum, suggesting species-specific endonuclease activity. Inhibiting endonucleases by incubating DNA-VLPs with diamidine-class MGBs increased their half-lives in mouse serum by more than 12 h, corroborated by protection against isolated DNase I and II. Our stabilization strategy was compatible with the functionalization of DNA-VLPs with HIV antigens, did not interfere with B-cell signaling activity of DNA-VLPs in vitro, and was nontoxic to B-cell lines. It was further found to be compatible with multiple wireframe DNA origami geometries and edge architectures. MGB protection is complementary to existing methods such as PEGylation and chemical cross-linking, offering a facile protocol to control DNase-mediated degradation rates for in vitro and possibly in vivo therapeutic and vaccine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.